...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Site-specific recombination between plasmids of Staphylococcus aureus.
【24h】

Site-specific recombination between plasmids of Staphylococcus aureus.

机译：金黄色葡萄球菌质粒之间的位点特异性重组。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Anomalous recombination between two similar but nonidentical, naturally occurring penicillinase plasmids, pI258 and pI524, leading to duplication and deletion of the beta-lactamase locus, is described. Physical mapping of these plasmids by heteroduplex and restriction analysis revealed that the beta-lactamase loci were homologous and in inverted orientation with respect to one another and that their respective locations were separated by a short region of homology. This intervening region of homology included one copy of a segment that was repeated on pI524 in inverted orientation at a distance of 2.2 kilobase pairs and contained a recognition sequence for a site-specific, rec-independent recombination function that caused reversible inversion of this segment on pI524. It is proposed that site-specific, intermolecular recombination involving this repeated sequence was responsible for the observed results.
机译：描述了两个相似但不相同的天然青霉素酶质粒pI258和pI524之间的异常重组，导致β-内酰胺酶基因座的复制和缺失。通过异源双链和限制性酶切分析对这些质粒进行的物理作图表明，β-内酰胺酶基因座是同源的，彼此之间呈倒置方向，它们各自的位置被短的同源区域隔开。此同源性的中间区域包括一个片段的一个拷贝，该片段在pI524上以2.2碱基对的间隔以反向定向重复，并包含一个位点特异性，独立于rec的重组功能的识别序列，该识别功能导致该片段的可逆转化。 pI524。有人提出，涉及该重复序列的位点特异性分子间重组是观察到的结果的原因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号