The protein compositions of the outer membranes of various Vibrio cholerae strains, belonging to both biotypes (El Tor and classical) and both serotypes (Ogawa and Inaba), were analyzed by electrophoresis on polyacrylamide slab gels in the presence of sodium dodecyl sulfate. All these strains contained a major protein band of molecular weight 48,000. But they differed in the composition and proportions of minor proteins. The outer membrane protein profile was influenced by the growth medium. A protein band of molecular weight 15,000 was observed in the outer membrane when V. cholerae Ogawa 395 (classical) was grown in peptone-water, whereas a protein of molecular weight 68,000 appeared when it was grown in the synthetic medium. Under anaerobic growth conditions the proportion of the 13,000-molecular-weight protein was greatly enhanced. The outer membrane contained heat-modifiable proteins, as the protein bands with molecular weights 41,000 and 37,000 disappeared when membrane proteins were disaggregated in sodium dodecyl sulfate at or above 60 degrees C. The antisera to the outer membrane proteins of V. cholerae Ogawa 395 (classical) produced immunoprecipitation to the outer membrane proteins of both biotypes and both serotypes. Also, the antisera agglutinated bacteria of both biotypes and both serotypes, suggesting the presence of a common protein antigen in the outer membrane of V. cholerae.
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机译：在十二烷基硫酸钠存在下，通过聚丙烯酰胺平板凝胶电泳，分析了分别属于生物型（El Tor和经典型）和两种血清型（Ogawa和Inaba）的霍乱弧菌菌株外膜的蛋白质组成。所有这些菌株均包含分子量为48,000的主要蛋白带。但是它们在次要蛋白质的组成和比例上有所不同。外膜蛋白概况受生长培养基的影响。在蛋白ept水中生长霍乱弧菌小川395时，在外膜上观察到分子量为15,000的蛋白质带，而在合成培养基中生长时，则出现了分子量为68,000的蛋白质。在厌氧生长条件下，13,000分子量蛋白的比例大大提高。外膜含有可热修饰的蛋白，当膜蛋白在60摄氏度或以上的温度在十二烷基硫酸钠中分解时，分子量为41,000和37,000的蛋白带消失。霍乱弧菌小川395（Hogawa V. cholerae Ogawa 395）的抗血清常规）对两种生物型和两种血清型的外膜蛋白产生免疫沉淀。同样，两种生物型和两种血清型的抗血清凝集细菌，都表明霍乱弧菌外膜中存在共同的蛋白抗原。
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