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首页> 外文期刊>Journal of bacteriology >Two fep genes are required for ferrienterochelin uptake in Escherichia coli K-12.
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Two fep genes are required for ferrienterochelin uptake in Escherichia coli K-12.

机译:大肠杆菌K-12中铁蛋白螯合素的摄取需要两个fep基因。

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摘要

Escherichia coli mutants defective in the assimilation of iron from ferrienterochelin were isolated and characterized. One mutant was able to bind ferrienterochelin to its outer membrane but could not transport it into the cell. Complementation tests with lambda hybrid phage were employed to distinguish the defective gene, which we term fepB, from fepA, the structural gene for the outer membrane ferrienterochelin receptor protein. These same physiological and genetic tests were employed to tentatively classify several previously described fep mutants as carrying either fepA or fepB. The data demonstrate the existence of fepB and provide an explanation for previous difficulties in identifying fepB mutants.
机译:分离并鉴定了铁从费铁螯合蛋白中同化缺陷的大肠杆菌突变体。一个突变体能够将Ferrienterochelin与其外膜结合,但不能将其转运到细胞中。用lambda杂合噬菌体进行补充测试,以将有缺陷的基因(我们称为fepB)与fepA(外膜铁结合蛋白受体蛋白的结构基因)区分开。这些相同的生理和遗传学测试被用于将几个先前描述的fep突变体暂时分类为携带fepA或fepB。数据证明了fepB的存在,并为先前鉴定fepB突变体的困难提供了解释。

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