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首页> 外文期刊>Journal of bacteriology >Metabolic activities of isolated akinetes of the cyanobacterium Nostoc spongiaeforme.
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Metabolic activities of isolated akinetes of the cyanobacterium Nostoc spongiaeforme.

机译：蓝藻Nostoc海绵状分离的鱼的代谢活性。

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摘要

Intact akinetes (spores) of the cyanobacterium Nostoc spongiaeforme can be isolated free of vegetative cells and heterocysts. The akinetes remain viable for at least 2 weeks in distilled water. They do not germinate in water but do so readily when transferred subsequently to cyanobacterial growth medium. Isolated, nongerminating akinetes incorporated 35S from Na235SO4 into protein and lipid. Similar incorporation was observed when akinetes were isolated from old cultures (containing primarily akinetes) which were labeled with Na235SO4 for 4 to 5 h before isolation. The metabolic activities of isolated akinetes were therefore not a factitious response to the isolation procedure. Autoradiographs of radioactive akinetes showed that 35S was incorporated by virtually all akinetes, rather than by a small subpopulation of active cells. Akinetes consumed O2 in the dark and, in a dichlorophenyl dimethylurea-sensitive reaction, evolved O2 in the light. We conclude that akinetes are metabolically active under conditions in which germination does not occur.
机译：可以分离出没有营养细胞和异胞藻的蓝细菌Nostoc spongiaeforme的完整akinetes（孢子）。鱼在蒸馏水中可存活至少2周。它们不会在水中发芽，但随后转移到蓝细菌生长培养基中时很容易发芽。分离的，未发芽的鱼将来自Na235SO4的35S掺入蛋白质和脂质。当从旧培养物中分离出突蛋白（主要含有突蛋白）时，观察到了类似的掺入，在分离前用Na235SO4标记了4至5小时。因此，分离的秋葵的代谢活性不是对分离过程的人为反应。放射性骨的放射自显影照片显示，几乎所有骨都掺入了35S，而不是由活性细胞的一小部分亚群掺入。 Akinetes在黑暗中消耗O2，在对二氯苯基二甲基脲敏感的反应中，在光下释放O2。我们得出的结论是，在未发芽的条件下，鱼具有代谢活性。

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