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首页> 外文期刊>Journal of bacteriology >Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli.
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Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli.

机译：大肠杆菌对苯丙酸及其3-羟基衍生物的分解代谢。

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摘要

A number of laboratory strains and clinical isolates of Escherichia coli utilized several aromatic acids as sole sources of carbon for growth. E. coli K-12 used separate reactions to convert 3-phenylpropionic and 3-(3-hydroxyphenyl)propionic acids into 3-(2,3-dihydroxyphenyl)propionic acid which, after meta-fission of the benzene nucleus, gave succinate, pyruvate, and acetaldehyde as products. Enzyme assays and respirometry showed that all enzymes of this branched pathway were inducible and that syntheses of enzymes required to convert the two initial growth substrates into 3-(2,3-dihydroxyphenyl)propionate are under separate control. E. coli K-12 also grew with 3-hydroxycinnamic acid as sole source of carbon; the ability of cells to oxidize cinnamic and 3-phenylpropionic acids, and hydroxylated derivatives, was investigated. The lactone of 4-hydroxy-2-ketovaleric acid was isolated from enzymatic reaction mixtures and its properties, including optical activity, were recorded.
机译：大肠杆菌的许多实验室菌株和临床分离株利用几种芳族酸作为生长的唯一碳源。大肠杆菌K-12使用不同的反应将3-苯基丙酸和3-（3-羟苯基）丙酸转化为3-（2,3-二羟苯基）丙酸，苯核的间裂后得到琥珀酸，丙酮酸和乙醛为产物。酶测定和呼吸测定法表明该分支途径的所有酶都是可诱导的，并且将两种初始生长底物转化为3-（2,3-二羟基苯基）丙酸酯所需的酶的合成处于单独的控制之下。大肠杆菌K-12也以3-羟基肉桂酸作为唯一碳源生长。研究了细胞氧化肉桂酸和3-苯基丙酸以及羟基化衍生物的能力。从酶促反应混合物中分离出4-羟基-2-酮戊酸的内酯，并记录其性质，包括旋光性。

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