DNA repair in Saccharomyces cerevisiae: purification and characterization of apurinic endonucleases.

P R Armel; S S Wallace

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DNA repair in Saccharomyces cerevisiae: purification and characterization of apurinic endonucleases.

机译：酿酒酵母中的DNA修复：嘌呤内切核酸酶的纯化和鉴定。

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Five chromatographically distinct apurinic endonucleases (D1, D2, D3, D4, and E) were purified from Saccharomyces cerevisiae 234, 122, 1,000, 4,550, and 5,490-fold, respectively. All appeared to be class II apurinic endonucleases and were not contaminated with exonuclease or nonspecific endonuclease activities under the reaction conditions used. All had similar pH optima, but endonucleases D4 and E showed higher salt requirements and endonuclease D4 had a lower Mg2+ requirement for optimal activity than the other endonucleases. Endonuclease D4 also nicked OsO4-treated DNA. The molecular weights of the apurinic endonucleases as determined by glycerol gradient sedimentation analysis were 37,000, 49,000, and 10,000, for endonucleases E, D4, and D2, respectively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of samples of radioiodinated endonuclease E showed the presence of two proteins.

机译：从酿酒酵母234、122、1,000、4,550和5,490倍分别纯化了五个色谱上不同的紫质内切核酸酶（D1，D2，D3，D4和E）。在所使用的反应条件下，所有这些似乎都是II类嘌呤核酸内切酶，并且没有被核酸外切酶或非特异性核酸内切酶活性污染。所有这些都具有相似的最适pH，但是与其他核酸内切酶相比，核酸内切酶D4和E显示出更高的盐分需求，而核酸内切酶D4具有更低的Mg2 +以获得最佳活性。核酸内切酶D4也刻痕了OsO4处理的DNA。通过甘油梯度沉降分析确定的嘌呤核酸内切酶的分子量对于核酸内切酶E，D4和D2分别为37,000、49,000和10,000。放射性碘标记的核酸内切酶E样品的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示存在两种蛋白质。

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《Journal of bacteriology》 |1984年第3期|共8页
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P R Armel; S S Wallace;
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2. Purification and properties of the alkylation repair DNA glycosylase encoded the MAG gene from Saccharomyces cerevisiae. [J] . Bjoras M, Klungland A, Johansen RF, Biochemistry . 1995,第14期

机译：烷基化修复DNA糖基化酶的纯化和性质编码酿酒酵母的MAG基因。
3. Purification and characterization of the DNA binding domain of Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80 [J] . Sopko R., Stuart DT. Protein Expression and Purification . 2004,第1期

机译：酿酒酵母减数分裂特异性转录因子Ndt80 DNA结合结构域的纯化和表征
4. Measurement of DNA Repair Protein Apurinic/Apyrimidinic Endonuclease 1 (APE1) in Human Tissues by Liquid Chromatography/Tandem Mass Spectrometry with Isotope Dilution [C] . Pawel Jaruga, Guldal Kirkali, Prasad T. Reddy, ASMS Conference on Mass Spectrometry and Allied Topics . 2016

机译：用同位素稀释测量人体组织中DNA修复蛋白茴香蛋白/亚氨基酰胺内核酸酶1（APE1），同位素稀释
5. Biochemical and Functional Characterization of Rpa2 N-Terminal Phosphorylation During DNA Repair and Checkpoint Adaptation in Saccharomyces cerevisiae [D] . Wilson, Timothy Michael. 2018

机译：Rpa2 N末端磷酸化过程中酿酒酵母的DNA修复和检查点适应的生化和功能表征。
6. DNA repair in Saccharomyces cerevisiae: purification and characterization of apurinic endonucleases. [O] . P R Armel, S S Wallace 1984

机译：酿酒酵母中的DNA修复：嘌呤内切核酸酶的纯化和鉴定。
7. DNA repair in Saccharomyces cerevisiae: purification and characterization of apurinic endonucleases [O] . P R Armel, S S Wallace 1984

机译：酿酒酵母酿酒酵母中的DNA修复：纯化和表征膜的内切核酸酶
8. Studies of DNA Repair in Saccharomyces Cerevisiae. I. Characterization of a New Allele of RAD6. II. Investigation of Events in the First Cell Cycle after DNA Damage [R] . Douthwright-Fasse, J. A. 1979

机译：酿酒酵母中DNa修复的研究。一，RaD6新等位基因的特征。 II。 DNa损伤后第一个细胞周期事件的调查

DNA repair in Saccharomyces cerevisiae: purification and characterization of apurinic endonucleases.

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