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首页> 外文期刊>Journal of bacteriology >Integration and excision of pMC7105 in Pseudomonas syringae pv. phaseolicola: involvement of repetitive sequences.
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Integration and excision of pMC7105 in Pseudomonas syringae pv. phaseolicola: involvement of repetitive sequences.

机译：丁香假单胞菌pv中pMC7105的整合和切除。菜豆：涉及重复序列。

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The site for integration of pMC7105 into the chromosome of Pseudomonas syringae pv. phaseolicola has been mapped to a 2.6-kilobase-pair (kb) Bg/II-EcoRI fragment on this 150-kb indigenous plasmid. Selected excision plasmids resulting from imprecise excision of pMC7105 were used to identify one of the plasmid-chromosome juncture fragments and to characterize the mechanism of recombination from the chromosome. A 14.2-kb BamHI plasmid-chromosome juncture fragment has been identified in pEX8060 (234 kb), an excision plasmid which carries approximately 90 kb of chromosomal sequences to the left of the site of integration. This fragment contains a portion of the 2.6-kb Bg/II-EcoRI fragment as well as chromosomal sequences. Blot hybridization with a probe made from selected fragments of pMC7105 revealed three distinct repetitive sequences, RS-I, RS-II, and RS-III, on this plasmid. The 2.6-kb fragment, to which the site of integration maps, also contains RS-II. Five copies of RS-II are present in pMC7105, and more than 20 copies are present in the chromosome. Eight small excision plasmids were shown to result from recombination among fragments of pMC7105 that contain common repetitive sequences. The results indicate that integration and excision of pMC7105 occur through general recombination at homologous repetitive sequences.
机译：pMC7105整合到丁香假单胞菌pv染色体中的位点。菜豆已经被定位到这个150 kb本地质粒上的2.6碱基对（kb）Bg / II-EcoRI片段。从不正确切除pMC7105产生的选定切除质粒被用于鉴定质粒-染色体接合片段之一，并表征从染色体重组的机制。已经在pEX8060（234 kb）中鉴定了一个14.2 kb BamHI质粒-染色体接合片段，这是一种切除质粒，在整合位点的左侧带有大约90 kb的染色体序列。该片段包含2.6-kb Bg / II-EcoRI片段的一部分以及染色体序列。用由pMC7105的选定片段制成的探针进行印迹杂交，在该质粒上发现了三个不同的重复序列，即RS-1，RS-II和RS-III。集成站点映射到的2.6kb片段也包含RS-II。 pMC7105中存在5个拷贝的RS-II，而染色体中存在20个以上的拷贝。已显示八个小型切除质粒是由含有共同重复序列的pMC7105片段之间的重组产生的。结果表明pMC7105的整合和切除是通过在同源重复序列处的一般重组发生的。

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