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首页> 外文期刊>Journal of bacteriology >Mutation spoT of Escherichia coli increases expression of the histidine operon deleted for the attenuator.
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Mutation spoT of Escherichia coli increases expression of the histidine operon deleted for the attenuator.

机译：大肠杆菌的spoT突变增加了减毒剂缺失的组氨酸操纵子的表达。

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摘要

F'-episomes carrying the Salmonella typhimurium wild-type or attenuator-deleted histidine (his) operons were introduced into Escherichia coli strains containing relA or spoT single and double mutations known to affect guanosine 3'-diphosphate 5'-diphosphate (ppGpp) and guanosine 3'-triphosphate 5'-diphosphate (pppGpp) levels. Expression of the his operon and expression of the gene for 6-phosphogluconate dehydrogenase (gnd) were measured during balanced growth in amino acid-rich and minimal media. The data were consistent with the interpretation that ppGpp is a positive effector of his operon expression, whereas pppGpp is not an essential effector. The conclusion that his operon expression is maximally stimulated at a lower than maximum intracellular ppGpp concentration was further confirmed. Neither ppGpp nor pppGpp appeared to influence gnd gene expression. The metabolic regulation of the E. coli his operon was found to be similar to the ppGpp-meidated metabolic regulation of the S. typhimurium his operon.
机译：将携带鼠伤寒沙门氏菌野生型或减毒剂缺失的组氨酸（his）操纵子的F'-附加异构体引入大肠杆菌菌株，该菌株含有relA或spoT单和双突变，已知会影响鸟苷3'-二磷酸5'-二磷酸（ppGpp）和鸟苷3'-三磷酸5'-二磷酸（pppGpp）水平。在富含氨基酸的培养基和基本培养基中平衡生长期间，测定了His操纵子的表达和6-磷酸葡萄糖酸脱氢酶（gnd）的基因的表达。数据与以下解释一致：ppGpp是其操纵子表达的阳性效应子，而pppGpp不是必需的效应子。进一步证实了其操纵子表达在低于最大细胞内ppGpp浓度时得到最大刺激的结论。 ppGpp和pppGpp都没有影响gnd基因的表达。发现他的操纵子大肠杆菌的代谢调控与他的操纵子鼠伤寒沙门氏菌的ppGpp介导的代谢调控相似。

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