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首页> 外文期刊>Journal of bacteriology >Physical mapping of beta-converting and gamma-nonconverting corynebacteriophage genomes.
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Physical mapping of beta-converting and gamma-nonconverting corynebacteriophage genomes.

机译：转化β和不转化γ的棒状噬菌体基因组的物理图谱。

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Deoxyribonucleic acids (DNAs) from wild-type and mutant strains of beta-converting and gamma-nonconverting corynebacteriophages were isolated and physically characterized. The data obtained from DNA heteroduplexes, restriction enzyme banding profiles, and restriction maps reinforce the conclusion that beta and gamma phages are very closely related. The major physical differences seen in the DNA heteroduplexes are a small substitution bubble and one or two insertions which are present on the gamma phage genome. The insertions account for the differences in the genome sizes of beta and gamma phages, and with the substitution they are responsible for most of the differences in the restriction endonuclease profiles and maps of the corynebactriophage genomes, two special sites and the DNA fragments carrying them were identified. These were the cohesive (cos) sites and the specific attachment (attP) site of the vegetative phage genome. The behavior of these sites indicated that the transition of phage DNA from the vegetative to the prophage state involves the circularization of vegetative DNA through the cos sites and its integration into the bacterial chromosome via the attP site. The mechanism of corynebacteriophage integration was similar to that employed by Escherichia coli phage gamma. From the data assembled the physical and genetic maps of beta and gamma phage were oriented with respect to one another. The extensive similarity in their maps provides additional confirmation of a close evolutionary relationship.
机译：从野生型和突变的β-转化和γ-非转化棒状噬菌体菌株中脱氧核糖核酸（DNA）被分离并进行了物理表征。从DNA异源双链体，限制性酶带谱和限制性酶切图谱获得的数据进一步证实了β和γ噬菌体密切相关的结论。在DNA异源双链体中看到的主要物理差异是一个小的置换气泡和一个或两个在γ噬菌体基因组中存在的插入。插入解释了β和γ噬菌体的基因组大小的差异，并且通过替换它们引起了限制性内切核酸酶谱和棒状噬菌体基因组图谱，两个特殊位点以及携带它们的DNA片段的大多数差异。确定。这些是植物噬菌体基因组的内聚位点和特异附着位点。这些位点的行为表明，噬菌体DNA从营养状态到噬菌体状态的转变涉及营养DNA通过cos位点的环化以及通过attP位点整合到细菌染色体中。棒状噬菌体整合的机制与大肠杆菌噬菌体γ所采用的机制相似。根据收集的数据，β和γ噬菌体的物理图谱和遗传图谱彼此相对。他们在地图中的广泛相似性进一步证实了紧密的进化关系。

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