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首页> 外文期刊>Journal of bacteriology >Role of L-lysine-alpha-ketoglutarate aminotransferase in catabolism of lysine as a nitrogen source for Rhodotorula glutinis.
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Role of L-lysine-alpha-ketoglutarate aminotransferase in catabolism of lysine as a nitrogen source for Rhodotorula glutinis.

机译:L-赖氨酸-α-酮戊二酸氨基转移酶在赖氨酸分解代谢中的作用,作为赖氨酸红球菌的氮源。

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摘要

Wild-type and saccharopine dehydrogenaseless mutant strains of Rhodotorula glutinis grew in minimal medium containing lysine as the sole nitrogen source and simultaneously accumulated, in the culture supernatant, large amounts of a product identified as alpha-aminoadipic-delta-semialdehyde. The saccharopine dehydrogenase and pipecolic acid oxidase levels remained unchanged in wild-type cells grown in the presence of ammonium or lysine as the nitrogen source. Lysine-alpha-ketoglutarate aminotransferase activity was demonstrated in ammonium-grown cells. This activity was depressed in cells grown in the presence of lysine as the sole source of nitrogen.
机译:Rhodotorula glutinis的野生型和无蔗糖脱氢酶突变菌株在含有赖氨酸作为唯一氮源的基本培养基中生长,并同时在培养上清液中积累了大量被鉴定为α-氨基己二-δ-半醛的产物。在以铵或赖氨酸为氮源的条件下生长的野生型细胞中,糖精脱氢酶和胡椒酸氧化酶的水平保持不变。在铵生长的细胞中证明了赖氨酸-α-酮戊二酸氨基转移酶活性。在赖氨酸作为唯一氮源存在下生长的细胞中,这种活性受到抑制。

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