Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid.

M Russel; P Model

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Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid.

机译：用克隆在质粒上的无活性基因替代大肠杆菌的fip基因。

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To determine whether the fip gene of Escherichia coli, which is required for filamentous phage assembly, is required for cell viability, we replaced the chromosomal copy of the gene with an inactive copy introduced on a plasmid. We found that the fip gene is dispensable. The method we devised, which should be generally useful, was also tested with an inactivated rho gene. As expected, the rho gene is essential.

机译：为了确定是否有丝状噬菌体组装所需的大肠杆菌fip基因对于细胞活力而言是必需的，我们用导入质粒的无活性拷贝替换了该基因的染色体拷贝。我们发现fip基因是可有可无的。我们设计的方法通常应该是有用的，还用灭活的rho基因进行了测试。不出所料，rho基因至关重要。

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《Journal of bacteriology》 |1984年第3期|共6页
作者
M Russel; P Model;
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中图分类微生物学;
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6. Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid. [O] . M Russel, P Model 1984

机译：用克隆在质粒上的无活性基因替代大肠杆菌的fip基因。
7. Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid [O] . M Russel, P Model 1984

机译：在质粒上克隆的非活性基因替换大肠杆菌的FIP基因

Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid.

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