...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Lipopolysaccharide-deficient, bacteriophage-resistant mutants of Escherichia coli K-12.
【24h】

Lipopolysaccharide-deficient, bacteriophage-resistant mutants of Escherichia coli K-12.

机译：大肠杆菌K-12的脂多糖不足，耐噬菌体突变体。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacteriophage-resistant mutants isolated and classified in a previous study were examined for alterations in their lipopolysaccharide (LPS) composition, and properties likely to be affected by alterations in LPS composition were studied. It was found that many of the mutants of the Ktw (K2-resistance), Ttk (T2, T4, or K19 resistance), Bar (bacteriophage), Wrm (wide-range mutants), and miscellaneous resistance groups were altered in their response to a series of antibiotics and to two LPS-specific bacteriophages, C21 and U3. Furthermore, many of the bacteriophages to which these mutants were resistant adsorbed to LPS preparations. By direct sugar analysis of the mutant LPS preparations, it was shown that the mutants fitted into six distinct classes, which are readily derived from LPS core with a structure resembling that of Salmonella or Escherichia coli O100. A number of the mutants were shown to map between pyrE and mtl, which has been previously shown to be the site of a cluster of rfa genes in both Salmonella and E. coli. Outer membrane protein composition was studied in the above mutants using polyacrylamide gel electrophoresis. Some strains were shown to have alterations in the amount of major proteins. The nature of the bacteriophage receptors involved and the alterations leading to resistance are discussed.
机译：检查了在先前研究中分离和分类的抗噬菌体突变体的脂多糖（LPS）组成的变化，并研究了可能受LPS组成变化影响的特性。发现许多Ktw（K2抗性），Ttk（T2，T4或K19抗性），Bar（噬菌体），Wrm（大范围突变体）和杂种抗性组的突变体的反应均发生了改变。一系列抗生素和两个LPS特异性噬菌体C21和U3。此外，这些突变体对其具有抗性的许多噬菌体被LPS制品吸附。通过对突变的LPS制品的直接糖分析，表明该突变体分为六类，它们很容易衍生自具有类似于沙门氏菌或大肠杆菌O100的结构的LPS核心。显示许多突变体在pyrE和mt1之间作图，先前已证明它们是沙门氏菌和大肠杆菌中rfa基因簇的位点。使用聚丙烯酰胺凝胶电泳在上述突变体中研究了外膜蛋白组成。某些菌株显示出主要蛋白质含量的变化。讨论了所涉及的噬菌体受体的性质和导致耐药性的改变。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号