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首页> 外文期刊>Journal of bacteriology >Protein synthesis during transition and stationary phases under glucose limitation in Saccharomyces cerevisiae.
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Protein synthesis during transition and stationary phases under glucose limitation in Saccharomyces cerevisiae.

机译：酿酒酵母在葡萄糖限制下的过渡期和固定期蛋白质合成。

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摘要

Metabolic changes have been investigated during continuous growth of yeast cells inoculated in glucose-containing medium until the cells entered the stationary phase in response to glucose exhaustion. Well in advance of glucose exhaustion, a transition phase was observed, characterized by a decrease in the growth rate and a progressive reduction of protein and RNA accumulation. Two-dimensional gel analysis of the proteins synthesized during this stage showed that the pattern of proteins remained similar to that of log-phase cells. When the cells entered the stationary phase, protein accumulation was 10% of that in log-phase cells, and incorporation of labeled RNA precursor was undetectable. Analysis of protein synthesis gave evidence that the synthesis of 95% of the proteins present in log-phase cells was arrested in stationary-phase cells. Among the 20 proteins whose synthesis continues throughout the stationary phase were identified actin, aldehyde dehydrogenase, enolase, hexokinase, glyceraldehyde-3-phosphate dehydrogenase, and five heat shock proteins. In addition, the synthesis of six new proteins was observed. The occurrence of these new proteins in stationary-phase cells is presumed to result from the release of carbon catabolite repression due to glucose exhaustion.
机译：已经研究了在含葡萄糖培养基中接种的酵母细胞连续生长期间的代谢变化，直到细胞响应葡萄糖耗尽而进入静止期。在葡萄糖耗尽之前，已经观察到过渡阶段，其特征在于生长速率降低以及蛋白质和RNA积累的逐渐减少。在此阶段合成的蛋白质的二维凝胶分析表明，蛋白质的模式与对数期细胞相似。当细胞进入固定相时，蛋白质积累是对数期细胞的10％，并且无法检测到标记的RNA前体的掺入。对蛋白质合成的分析提供了证据，证明对数期细胞中存在的95％蛋白质的合成被阻滞在固定期细胞中。在其合成在整个固定相中持续进行的20种蛋白质中，鉴定出了肌动蛋白，醛脱氢酶，烯醇酶，己糖激酶，3-磷酸甘油醛脱氢酶和5种热激蛋白。另外，观察到了六种新蛋白质的合成。推测这些新蛋白在固定相细胞中的发生是由于葡萄糖耗尽导致碳分解代谢物阻遏的释放。

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