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首页> 外文期刊>Journal of bacteriology >Nontoxic lipopolysaccharide from Rhodopseudomonas sphaeroides ATCC 17023.
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Nontoxic lipopolysaccharide from Rhodopseudomonas sphaeroides ATCC 17023.

机译:来自球形红假单胞菌ATCC 17023的无毒脂多糖。

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摘要

Chemical analysis of the lipopolysaccharide from Rhodopseudomonas sphaeroides ATCC 17023, isolated by the phenol-chloroform-petroleum ether method, revealed the presence of glucuronic acid, 2-keto-3-deoxyoctonate, threonine, and phosphorus in the polysaccharide moiety. The lipid A component contained glucosamine, glucosamine phosphate, amide-bound 3-oxotetradecanoic acid and 3-hydroxytetradecanoic acid, and ester-bound 3-hydroxydecanoic acid and 7-tetradecenoic acid. Structural similarity of the lipid A from R. sphaeroides ATCC 17023 to enterobacterial lipid A is indicated by the existence of a serological cross-reaction occurring between the lipid A from R. sphaeroides ATCC 17023 and that from Salmonella minnesota R595. The lipopolysaccharide and lipid A of R. sphaeroides, however, were found to be neither toxic in mice nor pyrogenic in rabbits.
机译:通过球形苯酚-氯仿-石油醚方法分离的球形红假单胞菌ATCC 17023的脂多糖的化学分析显示,多糖部分中存在葡萄糖醛酸,2-酮-3-脱氧辛酸,苏氨酸和磷。脂质A组分包含葡糖胺,葡糖胺磷酸,酰胺键合的3-氧代十四烷酸和3-羟基十四烷酸,以及酯键合的3-羟基癸酸和7-十四烯酸。来自球形红球菌ATCC 17023的脂质A与肠道细菌脂质A的结构相似性由球形红球菌ATCC 17023的脂质A与明尼苏达沙门氏菌R595的脂质A之间发生了血清学交叉反应来表明。然而,发现球形红球菌的脂多糖和脂质A对小鼠无毒,对兔子无热原。

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