Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules.

E Brickman; T J Silhavy; P J Bassford; H A Shuman; J R Beckwith

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Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules.

机译：大肠杆菌基因lacZ内用于形成活性杂合β-半乳糖苷酶分子的位点。

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We describe the genetic analysis of 21 Escherichia coli strains in which the amino-terminal sequence of beta-galactosidase has been removed and replaced by an amino-terminal sequence from one or another of the proteins involved in maltose transport. Genetic mapping of the lacZ end of these fused genes indicates that only those fusions in which fewer than 41 amino acids are removed from the amino-terminal sequence of beta-galactosidase result in enzymatically active molecules. Within the region between amino acid 17 and amino acid 41 there are at least four or five sites where enzymatically active hybrid proteins can be formed.

机译：我们描述了21个大肠杆菌菌株的遗传分析，其中β-半乳糖苷酶的氨基末端序列已被去除，并被一个或另一个参与麦芽糖运输的蛋白质的氨基末端序列取代。这些融合基因的lacZ末端的遗传图谱表明，只有那些从β-半乳糖苷酶的氨基末端序列中去除少于41个氨基酸的融合物才会产生酶活性分子。在氨基酸17和氨基酸41之间的区域内，存在至少四个或五个可以形成酶活性杂合蛋白的位点。

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《Journal of bacteriology》 |1979年第1期|共6页
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E Brickman; T J Silhavy; P J Bassford; H A Shuman; J R Beckwith;
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中图分类微生物学;
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1. An allolactose trapped at the lacZ beta-galactosidase active site with its galactosyl moiety in a H-4(3) conformation provides insights into the formation, conformation, and stabilization of the transition state [J] . Wheatley Robert W., Huber Reuben E. Biochemistry and Cell Biology . 2015,第6期

机译：捕获在lacZβ-半乳糖苷酶活性位点的半乳糖及其半乳糖基部分呈H-4（3）构型，可洞悉过渡态的形成，构型和稳定化
2. Importance of Arg-599 of beta-galactosidase (Escherichia coli) as an anchor for the open conformations of Phe-601 and the active-site loop [J] . Megan L. Dugdale, Michael L. Vance, Robert W. Wheatley, Biochemistry and Cell Biology . 2010,第6期

机译：β-半乳糖苷酶（大肠杆菌）的Arg-599作为Phe-601开放构象和活性位点环的锚点的重要性
3. A lacZ-pbpB gene fusion coding for an inducible hybrid protein that recognizes localized sites in the inner membrane of Escherichia coli. [J] . J A Ayala, J Plá, L R Desviat, Journal of bacteriology . 1988,第8期

机译：lacZ-pbpB基因融合体，编码可诱导的杂交蛋白，可识别大肠杆菌内膜中的定位位点。
4. An Efficient Site-directed Mutagenesis Method in vivo in Escherichia Coli [C] . Muqing Qiu International Conference on Mechanical Engineering and Intelligent Systems . 2012

机译：在大肠杆菌中的体内有效的站点定向诱变方法
5. Fate and transport of Escherichia coli within sediment basins on active construction sites. [D] . Tempel, Elizabeth A. 2011

机译：活跃建筑工地上沉积池内大肠杆菌的命运和运输。
6. Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules. [O] . E Brickman, T J Silhavy, P J Bassford Jr, 1979

机译：大肠杆菌基因lacZ内用于形成活性杂合β-半乳糖苷酶分子的位点。
7. Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules [O] . E Brickman, T J Silhavy, P J Bassford, 1979

机译：基因Lacz内的位点，用于形成活性杂交β-半乳糖苷酶分子
8. "Studies on the nature of the genetic material transferred during conjugation in Escherichia coli." and "Genetic and biochemical studies on the cell-free formation of tryptophan synthetase in Escherichia coli." [R] . 1963

机译：“在大肠杆菌结合过程中转移的遗传物质的性质研究。”和“在大肠杆菌中无色素形成色氨酸合成酶的遗传和生化研究”。

Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules.

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