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首页> 外文期刊>Journal of bacteriology >Iron supply to Escherichia coli by synthetic analogs of enterochelin.
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Iron supply to Escherichia coli by synthetic analogs of enterochelin.

机译：肠螯合素的合成类似物向大肠杆菌供应铁。

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摘要

Synthetic analogs of enterochelin (enterobactin) were tested for their ability to support the growth of Escherichia coli K-12 under iron-limiting conditions. The cyclic compound MECAM [1,3,5-N.N'; N"-tris-(2,3-dihydroxybenzoyl)-triamino-methylbenzene] and its N-methyl derivative Me3MECAM promoted growth, whereas the 2,3-dihydroxy-5-sulfonyl derivatives MECAMS and Me3MECAMS were inactive. The same results were obtained with TRIMCAM [1,3,5-tris(2,3-dihydroxybenzoylcarbamido)-benzene] and TRIMCAMS (the 2,3-dihydroxy-5-sulfonyl derivative of TRIMCAM). However, the sulfonic acid-containing linear compound LICAMS [1,5,10-N,N', N"-tris(5-sulfo-2,3-dihydroxybenzoyl)-triaza-decane] supported growth. In contrast, LIMCAMC, in which the sulfonyl groups at the five position of LICAMS are replaced by carboxyl groups at the four position, was inactive. The uptake of the active analogs required the functions specified by the fepB, fesB, and tonB genes. Surprisingly, growth promotion of mutants lacking the enterochelin receptor protein in the outer membrane was observed. Only MECAM protected cells against colicin B (which kills cells after entering at the enterochelin uptake sites) and transported Fe3+ at about half the enterochelin rate.
机译：测试了肠螯合素（肠杆菌素）的合成类似物在铁限制条件下支持大肠杆菌K-12生长的能力。环状化合物MECAM [1,3,5-N.N'; N“-三-（2,3-二羟基苯甲酰基）-三氨基甲基苯]及其N-甲基衍生物Me3MECAM促进生长，而2,3-二羟基-5-磺酰基衍生物MECAMS和Me3MECAMS没有活性。获得相同的结果与TRIMCAM [1,3,5-三（2,3-二羟基苯甲酰氨基甲酰氨基）-苯]和TRIMCAMS（TRIMCAM的2,3-二羟基-5-磺酰基衍生物）。然而，含磺酸的线性化合物LICAMS [1 ，5,10-N，N'，N“-三（5-磺基-2,3-二羟基苯甲酰基）-三氮杂癸烷]支持生长。相反，LIMCAMC是不活泼的，其中LICAMS的五个位置的磺酰基被羧基的四个位置取代。摄取活性类似物需要fepB，fesB和tonB基因指定的功能。令人惊讶地，观察到外膜中缺乏肠螯合素受体蛋白的突变体的生长促进。只有MECAM保护细胞免受大肠菌素B的侵害（在进入肠B素摄取部位后杀死细胞），并以大约一半的肠chel素速度转运Fe3 +。

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