...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Integration of R91-5::Tn501 into the Pseudomonas putida PPN chromosome and genetic circularity of the chromosomal map.
【24h】

Integration of R91-5::Tn501 into the Pseudomonas putida PPN chromosome and genetic circularity of the chromosomal map.

机译：R91-5 :: Tn501整合到恶臭假单胞菌PPN染色体和染色体图的遗传圆度中。

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Derivatives of the Pseudomonas aeruginosa plasmid R91-5, loaded with the transposon Tn501, were transferred to P. putida PPN. Over 90% of exconjugants, which arose at a frequency of ca. 10(-6) per donor cell, exhibited high-frequency (greater than 10(-2) per donor cell) polarized transfer of chromosomal markers. In one instance it was demonstrated by transduction that the plasmid had been inserted into a gene required for serine biosynthesis. The integrated nature of the plasmid in this and other P. putida (R91-5::Tn501) derivatives was supported by the failure to detect covalently closed circular DNA in these strains. The transfer origins of six different Hfr donors have been characterized genetically, and time-of-entry kinetics obtained from interrupted matings have enabled the construction of a circular genetic map 103 min in length and containing 35 markers. The genetic map of P. putida PPN shows significant differences in marker order to that of P. aeruginosa PAO.
机译：载有转座子Tn501的铜绿假单胞菌质粒R91-5的衍生物被转移到恶臭假单胞菌PPN中。超过90％的准分子偶联剂的出现频率约为每个供体细胞10（-6），表现出高频的染色体标记极化转移（每个供体细胞大于10（-2））。在一种情况下，通过转导证明该质粒已经插入到丝氨酸生物合成所需的基因中。质粒在该和其他恶臭假单胞菌（R91-5 :: Tn501）衍生物中的整合性质因未能检测到这些菌株中共价闭合的环状DNA而得到支持。六个不同的Hfr供体的转移起源已进行了遗传学表征，并且从中断交配获得的进入时间动力学特性使得能够构建长度为103分钟，包含35个标记的环状遗传图谱。恶臭假单胞菌PPN的遗传图谱显示标记顺序与铜绿假单胞菌PAO的标记顺序显着不同。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号