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首页> 外文期刊>Journal of bacteriology >Identification of a new insertion element, similar to gram-negative IS26, on the lactose plasmid of Streptococcus lactis ML3.
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Identification of a new insertion element, similar to gram-negative IS26, on the lactose plasmid of Streptococcus lactis ML3.

机译：在乳酸链球菌ML3的乳糖质粒上鉴定出一个类似于革兰氏阴性IS26的新插入元件。

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In Streptococcus lactis ML3, the lactose plasmid (pSK08) forms cointegrates with a conjugal plasmid (pRS01). It has been proposed that cointegration is mediated by insertion sequences (IS) present on pSK08 (D. G. Anderson and L.L. McKay, J. Bacteriol. 158:954-962, 1984). We examined the junction regions of the cointegrate pPW2 and the corresponding regions of pSK08 (donor) and pRS01 (target) and identified a new IS element on pSK08 (ISS1S) which was involved in and duplicated during formation of pPW2. ISS1S was 808 base pairs (bp) in size, had 18-bp inverted repeats (GGTTCTGTTGCAAAGTTT) at its ends, contained a single long open reading frame encoding a putative protein of 226 amino acids, and generated 8-bp direct repeats of target DNA during cointegrate formation. An iso-IS element, ISS1T, which is duplicated in some other cointegrate plasmids, was also found on pSK08. ISS1T was also 808 bp in size and was identical to ISS1S in sequence except for 4 bp, none of which altered the inverted repeats or amino acid sequence of the open reading frame. Comparison of ISS1 with gram-negative IS26 revealed strong homologies in size (820 bp), sequence of inverted repeats (GGCACTGTTGCAAA), size of direct repeats generated after cointegration (8 bp), and number, size, and amino acid sequence (44.5% identical) of the open reading of frame.
机译：在乳酸链球菌ML3中，乳糖质粒（pSK08）与接合质粒（pRS01）形成共整合体。已经提出，共整合是由pSK08上存在的插入序列（IS）介导的（D.G.Anderson和L.L.McKay，J.Bacteriol.158：954-962，1984）。我们检查了pPW2共整合的连接区域以及pSK08（供体）和pRS01（靶标）的相应区域，并在pSK08（ISS1S）上鉴定了一个新的IS元件，该元件参与了pPW2的形成并在复制过程中重复了。 ISS1S大小为808个碱基对（bp），在其末端具有18bp的反向重复序列（GGTTCTGTTGCAAAGTTT），包含一个单一的长开放阅读框，编码一个推测为226个氨基酸的蛋白质，并生成了8bp的靶DNA直接重复序列在协整过程中。在pSK08上也发现了一个iso-IS分子ISS1T，它在其他一些共整合质粒中重复存在。 ISS1T的大小也为808 bp，除了4 bp以外，其序列与ISS1S相同，都没有改变开放阅读框的反向重复序列或氨基酸序列。 ISS1与革兰氏阴性IS26的比较显示出大小（820 bp），反向重复序列（GGCACTGTTGCAAA），共整合后产生的直接重复序列（8 bp）以及数量，大小和氨基酸序列（44.5％）的高度同源性相同）的框架的开放阅读。

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