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首页> 外文期刊>Journal of bacteriology >Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea.
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Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea.

机译：丁香假单胞菌pv的0族和1族克隆的无毒力基因的分子表征。甘氨酸。

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摘要

A wide-host-range cosmid cloning vector, pLAFR3, was constructed and used to make cosmid libraries of partially digested Sau3A DNA from race 0 and race 1 of Pseudomonas syringae pv. glycinea. Two avirulence genes, avrB0 and avrC, cloned from race 0, elicited the hypersensitivity reaction (HR) on specific cultivars of soybean. Race 4 transconjugants containing avrB0 induced a dark brown necrotic HR within 24 h on the soybean cultivars Harosoy and Norchief, whereas race 4 transconjugants containing avrC induced a light brown necrotic HR within 48 h on the soybean cultivars Acme, Peking, Norchief, and Flambeau. An additional avirulence gene, avrB1, cloned from race 1, appeared to be identical to avrB0 from race 0. The avrB0 and avrC genes from race 0 were characterized by restriction enzyme mapping, Southern blot analysis, Tn5 transposon mutagenesis, and site-directed gene replacements. The effects of these three genes on the in planta bacterial growth of race 4 transconjugants have also been examined. The identification and cloning of avrB1 provides genetic evidence for a gene-for-gene interaction in the bacterial blight disease of soybean, as avrB1 from race 1 interacts with the soybean disease resistance locus, Rpg1.
机译：构建了宽宿主范围的粘粒克隆载体pLAFR3，并将其用于从丁香假单胞菌pv的0号和1号种族中部分消化的Sau3A DNA制备粘粒文库。甘氨酸。从第0种族克隆的两个无毒基因avrB0和avrC引发了对大豆特定品种的超敏反应（HR）。含有avrB0的第4种族转导结合剂在24 h内对大豆品种Harosoy和Norchief产生了深棕色坏死的HR，而含有avrC的第4种族的结合缀合物在48 h内对大豆品种Acme，Peking，Norchief和Flambeau引起了浅棕色的坏死HR。从种族1克隆的另一个无毒力基因avrB1似乎与来自种族0的avrB0相同。来自种族0的avrB0和avrC基因的特征在于限制性酶切图谱，Southern印迹分析，Tn5转座子诱变和定点基因替代品。还研究了这三个基因对第4族转导结合体在植物中细菌生长的影响。 avrB1的鉴定和克隆为大豆细菌性枯萎病中的基因对基因相互作用提供了遗传学证据，因为来自第1种的avrB1与大豆抗病基因座Rpg1相互作用。

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