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首页> 外文期刊>Journal of bacteriology >Role of host factors in the regulation of the enterotoxin B gene.
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Role of host factors in the regulation of the enterotoxin B gene.

机译：宿主因子在肠毒素B基因调控中的作用。

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摘要

The levels of staphylococcal enterotoxin B (SEB) produced by various naturally occurring toxinogenic strains of Staphylococcus aureus are highly variable. The SEB gene (seb) from a high-producer strain, S6, has previously been cloned and characterized. Cloning and nucleotide sequence analysis of the upstream region of the seb gene from DU4916 and COL (medium- and low-level toxin-producer strains, respectively) showed that their sequence was identical to that of the seb gene from strain S6. Strains carrying the cloned seb gene from DU4916 and COL produced similar levels of SEB protein and mRNA to those produced by strains carrying the cloned seb gene from strain S6. An RNA encoded by the delta-lysin gene (hld) has been shown to regulate the genes for a number of extracellular proteins, including SEB. Northern (RNA) blot analysis showed that variable levels of hld RNA were present in various SEB-producer strains, with the order being S6 greater than DU4916 greater than COL. Our results suggest that differences in host factor(s), including the hld RNA, are responsible for the production of different amounts of SEB by many naturally occurring strains.
机译：由各种自然产生的金黄色葡萄球菌产毒菌株产生的葡萄球菌肠毒素B（SEB）的水平高度可变。高产菌株S6的SEB基因（seb）先前已被克隆和鉴定。 DU4916和COL（分别为中，低水平产毒菌株）的seb基因上游区域的克隆和核苷酸序列分析表明，它们的序列与菌株S6的seb基因的序列相同。带有从DU4916和COL克隆的seb基因的菌株产生的SEB蛋白和mRNA水平与带有从菌株S6克隆的seb基因的菌株产生的SEB蛋白和mRNA的水平相似。已显示由δ-溶素基因（hld）编码的RNA可以调节包括SEB在内的许多细胞外蛋白的基因。 Northern（RNA）印迹分析表明，在各种SEB产生菌株中存在可变水平的hld RNA，顺序为S6大于DU4916大于COL。我们的结果表明，宿主因子（包括hld RNA）的差异是导致许多天然菌株产生不同量SEB的原因。

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