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首页> 外文期刊>Journal of bacteriology >Role of translation of the pheA leader peptide coding region in attenuation regulation of the Escherichia coli pheA gene.
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Role of translation of the pheA leader peptide coding region in attenuation regulation of the Escherichia coli pheA gene.

机译：pheA前导肽编码区的翻译在大肠杆菌pheA基因的衰减调节中的作用。

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In Escherichia coli, the expression of the pheA gene is regulated by attenuation of transcription. To study the molecular details of pheA attenuation, we introduced mutations in the pheA leader peptide coding region and analyzed their effects by using pheA promoter-lacZ gene transcription fusions (pheAp-lacZ). Mutations in the ribosome-binding site (pheAe1213) or in the translation initiation codon (pheAe24) of the pheA leader peptide coding region resulted in superattenuation of pheA expression. However, the presence of a stop codon upstream to the tandem phenylalanine codons (pheAe3334) led to an increase in the basal-level expression of pheA. This increase was further enhanced in the presence of prfA release factor mutant. The level of pheA expression in all three mutants was the same when cells were starved for phenylalanine. These results demonstrate that efficient translation of the pheA leader peptide coding region and the position of the ribosome on the leader transcript play decisive roles in the attenuation regulation of pheA.
机译：在大肠杆菌中，pheA基因的表达受转录减弱的调节。为了研究pheA减毒的分子细节，我们在pheA前导肽编码区中引入了突变，并通过使用pheA启动子-lacZ基因转录融合体（pheAp-lacZ）分析了它们的作用。 pheA前导肽编码区的核糖体结合位点（pheAe1213）或翻译起始密码子（pheAe24）中的突变导致pheA表达超衰减。但是，串联苯丙氨酸密码子（pheAe3334）上游的终止密码子的存在导致pheA的基础水平表达增加。在prfA释放因子突变体的存在下，这种增加被进一步增强。当细胞饿死苯丙氨酸时，所有三个突变体中的pheA表达水平相同。这些结果表明，pheA前导肽编码区的有效翻译和核糖体在前导转录本上的位置在pheA的减毒调控中起着决定性的作用。

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