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首页> 外文期刊>Journal of bacteriology >Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon.
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Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon.

机译：通过使用mini-Tn10转座子来定位基本的大肠杆菌基因：pdxJ操纵子。

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摘要

The mini-Tn10 transposon (delta 16 delta 17Tn10) confers tetracycline resistance. When inserted between a gene and its promoter, it blocks transcription and prevents expression of that gene. Tetracycline in the medium induces divergent transcription of the tetA and tetR genes within the transposon, and this transcription extends beyond the transposon in both directions into the bacterial genes. If the mini-Tn10 inserts between an essential bacterial gene and its promoter, the insertion mutation can cause conditional growth which is dependent on the presence of tetracycline. Two essential genes in adjacent operons of Escherichia coli have been detected by screening for tetracycline dependence among tetracycline-resistant insertion mutants. These essential genes are the era gene in the rnc operon and the dpj gene in the adjacent pdxJ operon. The pdxJ operon has not been described previously. It consists of two genes, pdxJ and dpj. Whereas the dpj gene is essential for E. coli growth in all media tested, pdxJ is not essential. The pdxJ gene encodes a protein required in the biosynthesis of pyridoxine (vitamin B6).
机译：迷你-Tn10转座子（δ16δ17Tn10）赋予四环素抗性。当插入基因及其启动子之间时，它会阻止转录并阻止该基因的表达。培养基中的四环素诱导转座子内tetA和tetR基因的不同转录，并且该转录在两个方向上延伸超过转座子进入细菌基因。如果mini-Tn10插入必需细菌基因及其启动子之间，则插入突变会导致条件生长，这取决于四环素的存在。通过筛选四环素抗性插入突变体中的四环素依赖性，已检测到大肠杆菌相邻操纵子中的两个必需基因。这些必需基因是rnc操纵子中的era基因和相邻pdxJ操纵子中的dpj基因。以前没有描述过pdxJ操纵子。它由两个基因pdxJ和dpj组成。尽管在所有测试的培养基中dpj基因对于大肠杆菌的生长都是必不可少的，但pdxJ并不是必需的。 pdxJ基因编码吡ido醇（维生素B6）的生物合成所需的蛋白质。

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