...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Early Initiation of Deoxyribonucleic Acid Replication and Shortening of Generation Time Associated with Inhibition of Lateral Wall Formation by Mecillinam
【24h】

Early Initiation of Deoxyribonucleic Acid Replication and Shortening of Generation Time Associated with Inhibition of Lateral Wall Formation by Mecillinam

机译：与美西利南抑制侧墙形成相关的脱氧核糖核酸复制的早期启动和生成时间的缩短

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The effects of mecillinam on the growth of rods of the pH-conditional morphology mutant MirM7 was studied. It has been found that mecillinam causes, coincident with transition to coccal shape, a balanced rise in the rate of viable count increase and the rate of macromolecular synthesis which lasts either until the cells enter a stationary growth phase or indefinitely, in the case of continuously diluted cultures. When the antibiotic is removed from cells which have already become coccoid, cells continue to grow at a faster rate until they resume the rod shape. No change in the per-cell rate of protein synthesis has been seen in untreated or mecillinam-treated cells before or after the change in growth rate. Studies with synchronously growing cells have shown that the antibiotic causes a shortening in the I period (initiation of deoxyribonucleic acid replication). Evaluation of the residual divisions in nalidixic acid-treated, exponential-phase cells has shown that mecillinam also shortens the D period (cell division). It is proposed that, in strain MirM7, inhibition of lateral wall elongation by the antibiotic allows the initiation of a new septum, though inhibition is still in progress. The initiation of a new septum is, in turn, responsible for both the early inibition of deoxyribonucleic acid replication and accelerated division. In the parental strain, MirA12, as well as in other sensitive gram-negative rods which divide, become cocci, and stop dividing after addition of the antibiotic, inhibition of lateral wall formation activates a feedback mechanism which prevents insertion of new septa (Satta et al., J. Bacteriol. >142:43-51, 1980). Consequently, no early initiation of deoxyribonucleic acid replication is observed, and the last division allowed by the antibiotic occurs in due time. This negative control is missing in MirM7.
机译：研究了美西林对pH条件形态学突变体MirM7杆生长的影响。已经发现，美西林可引起与向球菌形状的转变相伴的是，活菌数增加速率和大分子合成速率的平衡上升，持续直至细胞进入静止的生长阶段，或者无限期地持续，直到连续进入细胞。稀释的培养物。当从已经成为类球体的细胞中去除抗生素时，细胞继续以更快的速度生长，直到它们恢复杆状。在生长速率变化之前或之后，在未处理或经美西林处理的细胞中未观察到每细胞蛋白质合成速率的变化。细胞同步生长的研究表明，抗生素会导致I期缩短（脱氧核糖核酸复制开始）。对萘啶酸处理的指数期细胞中的残余分裂进行评估表明，美西林还可以缩短D期（细胞分裂）。有人提出，在MirM7菌株中，抗生素对侧壁伸长的抑制作用允许启动新的隔膜，尽管这种抑制作用仍在进行中。反过来，新间隔的开始既负责脱氧核糖核酸复制的早期感染，又负责加速分裂。在亲本菌株MirA12以及其他敏感的革兰氏阴性杆菌中，它们在添加抗生素后会分裂，变成球菌并停止分裂，抑制侧壁形成会激活一种反馈机制，从而阻止新隔垫的插入（Satta等等人，细菌学杂志，> 142： 43-51，1980）。因此，没有观察到脱氧核糖核酸复制的提早开始，并且抗生素允许的最后分裂发生在适当的时间。 MirM7中缺少此阴性对照。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号