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首页> 外文期刊>Journal of bacteriology >Genetic characterization of the inducible SOS-like system of Bacillus subtilis.
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Genetic characterization of the inducible SOS-like system of Bacillus subtilis.

机译：枯草芽孢杆菌可诱导的SOS样系统的遗传表征。

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The SOS-like system of Bacillus subtilis consists of several coordinately induced phenomena (e.g., cellular filamentation, prophage induction, and Weigle reactivation of UV-damaged bacteriophage) which are expressed after cellular insult such as DNA damage or inhibition of DNA replication. Mutagenesis of the bacterial chromosome and the development or maintenance of competence also appear to be involved in the SOS-like response in this bacterium. The genetic characterization of the SOS-like system has involved an analysis of (i) the effects of various DNA repair mutations on the expression of inducible phenomena and (ii) the tsi-23 mutation, which renders host strains thermally inducible for each of the SOS-like functions. Bacterial filamentation was unaffected by any of the DNA repair mutations studied. In contrast, the induction of prophage after thermal or UV pretreatment was abolished in strains carrying the recE4, recA1, recB2, or recG13 mutation. The Weigle reactivation of UV-damaged bacteriophage was also inhibited by the recE4, recA1, recB2, or recG13 mutation, whereas levels of Weigle reactivation were lower in strains which carried the uvrA42, polA5, or rec-961 mutation than in the DNA repair-proficient strain. Strains which carried the recE4 mutation were incapable of chromosomal DNA-mediated transformation, and the frequency of this event was decreased in strains carrying the recA1, recB2, or tsi-23 mutation. Plasmid DNA transformation efficiency was decreased only in strains carrying the tsi-23 mutation in addition to the recE4, recA1, or recB2 mutation. The results indicate that the SOS-like system of B. subtilis is regulated at different levels by two or more gene products. In this report, the current data regarding the genetic regulation of inducible phenomena are summarized, and a model is proposed to explain the mechanism of SOS-like induction in B. subtilis.
机译：枯草芽孢杆菌的SOS样系统由几种协同诱导的现象（例如，细胞丝化，噬菌体诱导和紫外线损伤的噬菌体的韦格尔（Weigle）活化）组成，这些现象在细胞损伤后表达，例如DNA损伤或DNA复制抑制。细菌染色体的诱变和能力的发展或维持也似乎与这种细菌的SOS样反应有关。 SOS样系统的遗传特征涉及以下方面的分析：（i）各种DNA修复突变对诱导现象表达的影响，以及（ii）tsi-23突变，这使得宿主菌株对每个类似SOS的功能。细菌丝化不受研究的任何DNA修复突变的影响。相反，在进行了recE4，recA1，recB2或recG13突变的菌株中，热或紫外线预处理后的噬菌体诱导被废除了。 recE4，recA1，recB2或recG13突变也抑制了紫外线损坏的噬菌体的Weigle激活，而携带uvrA42，polA5或rec-961突变的菌株的Weigle激活水平低于DNA修复-熟练应变。带有recE4突变的菌株不能进行染色体DNA介导的转化，并且在带有recA1，recB2或tsi-23突变的菌株中，此事件的频率降低。除recE4，recA1或recB2突变外，质粒DNA转化效率仅在携带tsi-23突变的菌株中降低。结果表明，枯草芽孢杆菌的SOS样系统受两个或多个基因产物的调节水平不同。在本报告中，总结了有关可诱导现象的遗传调控的当前数据，并提出了一个模型来解释枯草芽孢杆菌中类似SOS诱导的机制。

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