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首页> 外文期刊>Journal of bacteriology >Properties and regulation of glutamine synthetase from Rhodospirillum rubrum.
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Properties and regulation of glutamine synthetase from Rhodospirillum rubrum.

机译:红螺螺旋藻谷氨酰胺合成酶的性质和调控。

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Glutamine synthetase from Rhodospirillum rubrum was purified and characterized with respect to its pH optimum and the effect of Mg2+ on its active and inactive forms. Both adenine and phosphorus were incorporated into the inactive form of the enzyme, indicating covalent modification by AMP. The modification could not be removed by phosphodiesterase. Evidence for regulation of the enzyme by oxidation was obtained. Extracts from oxygen-treated cells had lower specific activities than did extracts from cells treated anaerobically. Glutamine synthetase activity was found to decrease in the dark in phototrophically grown cells; activity was recovered on re-illumination.
机译:纯化和鉴定了来自红球螺旋藻的谷氨酰胺合成酶,并针对其最适pH和Mg2 +对其活性和非活性形式的影响进行了表征。腺嘌呤和磷都掺入了酶的失活形式,表明被AMP共价修饰。该修饰不能被磷酸二酯酶去除。获得了通过氧化调节酶的证据。氧处理细胞的提取物比厌氧处理细胞的提取物具有更低的比活。发现在光养生长的细胞中,谷氨酰胺合成酶活性在黑暗中降低。重新照明后恢复活性。

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