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首页> 外文期刊>Journal of bacteriology >Accumulation of alpha,alpha-trehalose by Rhizobium bacteria and bacteroids.
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Accumulation of alpha,alpha-trehalose by Rhizobium bacteria and bacteroids.

机译：根瘤菌和类细菌对α，α-海藻糖的积累。

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Four strains of Rhizobium japonicum (61A76 and USDA 110, 123, and 138) were grown in eight different defined media. Regardless of the carbon or nitrogen source supplied, alpha, alpha-trehalose was the major carbohydrate (among mono- and disaccharides) accumulated by all four strains. After 7 to 9 days of growth, trehalose generally accounted for 90 to 100% of the mono- and disaccharides detected. None of the four strains would grow with trehalose as a carbon source, but the utilization of endogenous trehalose was demonstrated under carbon starvation conditions in water culture or when the carbon supply in a defined medium was exhausted. Under these conditions, a small amount of trehalose was lost from cells to the medium. In a survey of most of the serogroups of R. japonicum and several strains of other Rhizobium species, all strains tested were found to accumulate some trehalose. Trehalose concentrations varied widely; the highest concentration recorded was 41 micrograms/mg of dry weight. In all but six strains trehalose accounted for greater than 80% of the mono- and disaccharides in cells. Fast-growing strains of R. japonicum also accumulated small amounts trehalose. R. japonicum bacteroids also synthesized trehalose; the quantity in nodules varied in approximate correspondence to accumulation of trehalose by cultured bacteria. In young soybean nodules (29 days after planting), 45 to 80% of the trehalose was recovered in the cytosol. There were differences among R. japonicum strains in the retention of trehalose, and the proportion of trehalose retained by bacteroids increased with increasing plant age for all strains.
机译：在八种不同的定义培养基中培养了四株日本根瘤菌菌株（61A76和USDA 110、123和138）。不管所提供的碳源或氮源如何，α，α-海藻糖都是所有四个菌株积累的主要碳水化合物（单糖和双糖中的一种）。生长7至9天后，海藻糖通常占检测到的单糖和二糖的90％至100％。这四种菌株都不会以海藻糖为碳源生长，但在水培养中碳饥饿条件下或限定培养基中的碳供应耗尽时，证明了内源性海藻糖的利用。在这些条件下，少量海藻糖从细胞流失到培养基中。在对日本根瘤菌的大多数血清群和其他根瘤菌属的几种菌株进行的调查中，发现所有测试的菌株都积累了一些海藻糖。海藻糖浓度差异很大。记录的最高浓度为41微克/毫克干重。除六个菌株外，海藻糖占细胞中单糖和二糖的80％以上。快速生长的日本根瘤菌菌株也积累了少量海藻糖。 R. japonicum类杆菌也合成了海藻糖;结节中细菌的数量大致与培养细菌积累海藻糖有关。在年轻的大豆小结节中（种植后29天），在细胞质中回收了45％至80％的海藻糖。在日本血吸虫菌株之间，海藻糖的保留存在差异，并且所有菌株中类细菌保留的海藻糖的比例均随植物年龄的增加而增加。

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