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首页> 外文期刊>Journal of bacteriology >Pneumococcal Forssman antigen: enrichment in mesosomal membranes and specific binding to the autolytic enzyme of Streptococcus pneumoniae.
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Pneumococcal Forssman antigen: enrichment in mesosomal membranes and specific binding to the autolytic enzyme of Streptococcus pneumoniae.

机译：肺炎球菌Forssman抗原：弥散性增生中膜和与肺炎链球菌自溶酶的特异性结合。

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摘要

The choline-containing pneumococcal membrane teichoic acid (Forssman antigen) can be isolated with the membrane fractions of the bacteria. The small vesicle (mesosomal) fraction generated during the formation of protoplasts seems to be highly enriched in this material. Forssman antigen was identified in cell fractions on the basis of (i) radioactive choline label, (ii) autolysin-inhibitory activity, and (iii) the sedimentation profile in sucrose density gradients with and without detergent. A membrane teichoic acid could also be isolated from pneumococci grown in medium in which choline was replaced by ethanolamine as the nutritionally required amino alcohol. This material contained radioactive ethanolamine label and behaved similarly to the choline-containing membrane teichoic acid during centrifugation in detergent-containing and detergent-free density gradients. On the other hand, the material had only low autolysin-inhibitory activity. Binding of pure pneumococcal autolysin to micelles of purified Forssman antigen could be demonstrated by mixing these components in vitro and analyzing them by sucrose density gradients and by agarose chromatography. No binding could be observed between the pneumococcal enzyme and the micellar forms of either cardiolipin or polyglycerophosphate-type lipoteichoic acid isolated from Streptococcus lactis.
机译：含胆碱的肺炎球菌膜软骨蛋白（福斯曼抗原）可与细菌的膜级分分离。在原生质体形成过程中产生的小囊泡（内体）部分似乎高度富集了这种物质。根据（i）放射性胆碱标记，（ii）自溶素​​抑制活性和（iii）有或没有去污剂的蔗糖密度梯度中的沉降曲线，在细胞级分中鉴定了Forssman抗原。还可以从在培养基中生长的肺炎链球菌中分离出膜磷胆酸，在该培养基中，胆碱被乙醇胺替代为营养所需的氨基醇。该物质含有放射性乙醇胺标记，在含有去污剂的密度梯度和无去污剂的密度梯度离心过程中，其行为类似于含胆碱的膜磷胆酸。另一方面，该材料仅具有低的自溶素抑制活性。可以通过在体外混合这些成分并通过蔗糖密度梯度和琼脂糖色谱分析来证明纯净的肺炎球菌自溶素与纯化的Forssman抗原的胶束的结合。从肺炎链球菌分离的肺炎球菌酶和心磷脂或聚甘油磷酸型脂磷壁酸的胶束形式之间未观察到结合。

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