...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli.
【24h】

Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli.

机译：大肠杆菌的firA基因和firA200（Ts）等位基因的克隆和核苷酸序列。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Escherichia coli gene firA, previously reported to code for a small, histonelike DNA-binding protein, has been cloned and found to reside immediately downstream from skp, a gene previously identified as the firA locus. firA encodes a 36-kDa protein. The mutant firA200(Ts) allele was also cloned and shown to contain three mutations, each mutation giving rise to a single amino acid change. Partially purified wild-type FirA (from a firA+ strain) and mutant FirA [from a firA200(Ts) strain] proteins have amino-terminal sequences predicted from their common DNA sequences. Both proteins lack an N-terminal methionine. Modest overexpression of wild-type or mutant FirA restored wild-type growth to firA200(Ts) strains at 43 degrees C, whereas high-level expression of wild-type FirA was required for more complete suppression of the rifampin sensitivity of firA200(Ts) rpoB double mutants. High-level expression of mutant FirA did not suppress this rifampin sensitivity.
机译：已克隆出大肠杆菌基因firA，先前报道其编码小的组蛋白样DNA结合蛋白，并发现其直接位于skp下游，该基因先前被鉴定为firA基因座。 firA编码36 kDa的蛋白质。还克隆了突变体firA200（Ts）等位基因，显示其包含三个突变，每个突变引起一个氨基酸变化。部分纯化的野生型FirA（来自firA +菌株）和突变型FirA（来自firA200（Ts）菌株）蛋白具有从其共同DNA序列预测的氨基末端序列。两种蛋白质都缺少N端蛋氨酸。野生型或突变型FirA的适度过表达将野生型生长恢复到43摄氏度下的firA200（Ts）菌株，而野生型FirA的高水平表达则需要更完全地抑制firA200（Ts）的利福平敏感性rpoB双突变体。突变FirA的高水平表达不能抑制这种利福平敏感性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号