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首页> 外文期刊>Journal of bacteriology >Evidence that coupling sequences play a frequency-determining role in conjugative transposition of Tn916 in Enterococcus faecalis.
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Evidence that coupling sequences play a frequency-determining role in conjugative transposition of Tn916 in Enterococcus faecalis.

机译：在粪肠球菌中Tn916的共轭转座中，偶联序列起着决定频率作用的证据。

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摘要

The conjugative transposon Tn916 (encodes resistance to tetracycline), originally identified in Enterococcus faecalis, moves by an excision-insertion process in which the rate-limiting step is believed to be excision. Individual transposon-containing strains exhibit characteristic mating frequencies which range over several orders of magnitude; the basis of this phenomenon is addressed in the present study. We were able to generate independent single-copy insertions in identical target locations and with similar orientations within a plasmid hemolysin determinant (cylA); however, transposition from this site occurred at very different frequencies (10(-8) to 10(-4) per donor) depending on the individual isolate. DNA sequencing analyses showed that the coupling (junction) sequences differed between isolates and thus appeared to be responsible for differences in excision frequencies. Other experiments showed that inducible transcription into either end of the transposon had no significant effect on transfer.
机译：最初在粪肠球菌中鉴定出的共轭转座子Tn916（编码对四环素的抗性）通过切除插入过程移动，其中限速步骤被认为是切除。单个含有转座子的菌株表现出特征性的交配频率，其变化范围超过几个数量级。该现象的基础在本研究中得到解决。我们能够在质粒溶血素决定簇（cylA）中的相同靶位和相似方向上产生独立的单拷贝插入;但是，根据各个分离株的不同，从该位点发生的转座发生的频率也非常不同（每个供体为10（-8）至10（-4））。 DNA测序分析表明，分离物之间的偶联（连接）序列不同，因此似乎是造成切除频率差异的原因。其他实验表明，可诱导转录到转座子的任一端对转移没有明显影响。

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