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首页> 外文期刊>Journal of bacteriology >Assessment of Rhodopseudomonas sphaeroides chromatophore membrane asymmetry through bilateral antiserum adsorption studies.
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Assessment of Rhodopseudomonas sphaeroides chromatophore membrane asymmetry through bilateral antiserum adsorption studies.

机译：通过双边抗血清吸附研究评估球形红假单胞菌的色谱膜不对称性。

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摘要

The asymmetric structure of the Rhodopseudomonas sphaeroides chromatophore membrane was examined in detail by crossed immunoelectrophoresis techniques. Because these methods are quantitative and allow increased resolution and sensitivity, it was possible to analyze simultaneously the relative transmembrane distribution of a number of previously identified antigenic components. This was demonstrated by analysis of immunoglobulin samples that were adsorbed by preincubation with either isolated chromatophores or osmotically protected spheroplasts. The photochemical reaction center, the light-harvesting bacteriochlorophyll a-protein complex, the L-lactate dehydrogenase, and reduced nicotinamide adenine dinucleotide dehydrogenase (EC 1.6.99.3) were found to be exposed on the chromatophore surface (cytoplasmic aspect of the membrane within the cell). Other antigenic components were found to be exposed on the surface of spheroplasts (periplasmic aspect of the in vivo chromatophore membrane). Antigens with determinants expressed on both sides of the chromatophore membrane were also identified. Charge shift crossed immunoelectrophoresis confirmed the suggested amphiphilic character of the pigment-protein complexes and identified several additional amphiphilic membrane components.
机译：通过交叉免疫电泳技术详细检查了球形红假单胞菌色谱膜的不对称结构。因为这些方法是定量的，并且可以提高分辨率和灵敏度，所以可以同时分析许多先前鉴定的抗原成分的相对跨膜分布。通过对免疫球蛋白样品的分析证明了这一点，该样品是通过与分离的色谱或渗透保护的原生质球预孵育而吸附的。发现光化学反应中心，集光的细菌叶绿素a蛋白复合物，L-乳酸脱氢酶和还原烟酰胺腺嘌呤二核苷酸脱氢酶（EC 1.6.99.3）暴露于色谱表面（膜内膜的细胞质方面）。细胞）。发现其他抗原成分暴露在原生质球表面（体内染色体细胞膜的周质方面）。还鉴定了在染色体膜两侧均表达有决定簇的抗原。电荷转移交叉免疫电泳证实了色素-蛋白质复合物的建议两亲特性，并鉴定了几种其他的两亲膜成分。

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