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首页> 外文期刊>Journal of bacteriology >Biochemical genetics of tryptophan synthesis in Pseudomonas acidovorans.
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Biochemical genetics of tryptophan synthesis in Pseudomonas acidovorans.

机译：嗜酸假单胞菌中色氨酸合成的生化遗传学。

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Sixty independent tryptophan auxotrophs of Pseudomonas acidovorans were isolated and characterized for nutritional response to intermediates of the pathway, accumulation of intermediates, and levels of tryptophan-synthetic enzymes. Mutants for each of the seven proteins catalyzing the five steps of tryptophan synthesis were obtained. Transductional analysis established three unlinked chromosomal regions: trpE, trpGDC, and trpFBA. The order of the genes within the two clusters was not determined. The levels and enzymatic activities of wild-type and mutant strains indicated that trpE and trpGDC were repressed by tryptophan. In contrast, trpFBA was not derepressed significantly by starvation for tryptophan. The trpG mutants had an additional requirement for p-aminobenzoate, which suggested that anthranilate synthase subunit II also served as glutamine-binding protein in the analogous reaction catalyzed by p-aminobenzoate synthase. In addition, trpD mutants revealed the ability of P. acidovorans to degrade anthranilate via the beta-ketoadipate pathway.
机译：分离出六十个独立的嗜酸假单胞菌色氨酸营养缺陷型，并表征其对途径中间物的营养反应，中间物的积累和色氨酸合成酶的水平。获得了催化色氨酸合成的五个步骤的七个蛋白质中的每一个的突变体。转导分析建立了三个未链接的染色体区域：trpE，trpGDC和trpFBA。尚未确定两个簇中基因的顺序。野生型和突变菌株的水平和酶活性表明色氨酸抑制了trpE和trpGDC。相反，色氨酸的饥饿并未显着抑制trpFBA。 trpG突变体对对氨基苯甲酸酯有额外的要求，这表明在对氨基苯甲酸酯合酶催化的类似反应中，邻氨基苯甲酸合酶亚基II也可作为谷氨酰胺结合蛋白。此外，trpD突变体揭示了嗜酸假单胞菌通过β-酮己二酸酯途径降解邻氨基苯甲酸的能力。

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