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首页> 外文期刊>Journal of bacteriology >Metabolism of L-fucose and L-rhamnose in Escherichia coli: differences in induction of propanediol oxidoreductase.
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Metabolism of L-fucose and L-rhamnose in Escherichia coli: differences in induction of propanediol oxidoreductase.

机译：L-岩藻糖和L-鼠李糖在大肠杆菌中的代谢：丙二醇氧化还原酶诱导的差异。

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摘要

Escherichia coli is capable of growing on L-fucose or L-rhamnose as a sole source of carbon and energy. When grown under anaerobic conditions on either sugar, a nicotinamide adenine dinucleotide-linked L-lactaldehyde:propanediol oxidoreductase activity is induced. The functioning of this enzyme results in the regeneration of oxidized nicotinamide adenine dinucleotide. Conditions of induction of the enzyme activity were studied and were found to display different characteristics on each sugar. In the rhamnose-grown cells, the increase in enzyme activity detected under inducing conditions was accompanied by the synthesis of propanediol oxidoreductase, as measured by the appearance in the extracts of a protein that reacts with propanediol oxidoreductase antibodies. In contrast, in fucose-grown cells, the level of propanediol oxidoreductase as measured by enzyme antibody-reacting material was high under noninducing and inducing conditions. Thus, the increase in enzyme activity detected in going from noninducing to inducing conditions in fucose-grown cells did not depend on the appearance of the specific protein but on the activation of the propanediol oxidoreductase already present in the cells in an inactive form. The propanediol oxidoreductase of both homologous systems should consequently be regulated by different control mechanisms.
机译：大肠杆菌能够在L-岩藻糖或L-鼠李糖上生长，是唯一的碳和能量来源。当在任一糖上在厌氧条件下生长时，会诱导烟酰胺腺嘌呤二核苷酸连接的L-丙醛：丙二醇氧化还原酶活性。该酶的功能导致氧化的烟酰胺腺嘌呤二核苷酸的再生。研究了诱导酶活性的条件，发现在每种糖上显示出不同的特性。在鼠李糖生长的细胞中，在诱导条件下检测到的酶活性的增加伴随着丙二醇氧化还原酶的合成，这是通过与丙二醇氧化还原酶抗体反应的蛋白质提取物中的出现来衡量的。相反，在岩藻糖生长的细胞中，通过酶抗体反应材料测得的丙二醇氧化还原酶水平在非诱导和诱导条件下较高。因此，在岩藻糖生长的细胞中，从非诱导状态转变为诱导状态时检测到的酶活性的增加，并不取决于特定蛋白质的外观，而是取决于已经以非活性形式存在于细胞中的丙二醇氧化还原酶的活化。因此，两个同源系统的丙二醇氧化还原酶应通过不同的控制机制进行调节。

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