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首页> 外文期刊>Journal of bacteriology >Postreplication repair of ultraviolet-irradiated transforming deoxyribonucleic acid in Bacillus subtilis.
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Postreplication repair of ultraviolet-irradiated transforming deoxyribonucleic acid in Bacillus subtilis.

机译：枯草芽孢杆菌中紫外线照射的转化脱氧核糖核酸的复制后修复。

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Repair of ultraviolet-irradiated transforming deoxyriboinucleic acid (DNA) in several strains of Bacillus subtilis was studied in order to determine the effects of excision repair and postreplication repair on transformation. Two mutations that cause a Uvr- and phenotype (uvr-1 and uvr-42) were shown to have strikingly different effects on repair of ultraviolet-irradiated transforming DNA. Genetic and kinetic evidence is presented to show that integrated DNA was apparently repaired by both excision and postreplication repair in wild-type and in uvr-1 recipients, although the latter excise pyrimidine dimers very slowly. In uvr-42 mutants, which are defective in incision at pyrimidine dimers, dimer-containing DNA was integrated. Postreplication repair apparently saved uvr-42 recipient cells from the lethal effects of integrated dimers, but the recombination events accompanying postreplication repair greatly reduced the linkage between closely linked genetic markers in the donor DNA. Repair of transforming DNA in a recG recipient, which does excision repair but not postreplication repair, was nearly as efficient as in wild-type cells. However, in this recipient linkage was altered only slightly, if at all, compared with wild-type cells. The apparent reduction in size of integrated regions of ultraviolet-irradiation transforming DNA probably results mainly from postreplication repair of larger integrated regions.
机译：为了确定切除修复和复制后修复对转化的影响，研究了几种枯草芽孢杆菌菌株中紫外线照射的转化脱氧核糖核酸（DNA）的修复。两种引起Uvr和表型的突变（uvr-1和uvr-42）显示出对紫外线照射的转化DNA修复具有显着不同的作用。遗传和动力学证据表明，在野生型和uvr-1受体中，切除和复制后修复均可明显修复整合的DNA，尽管后者的嘧啶二聚体非常缓慢。在嘧啶二聚体切口缺陷的uvr-42突变体中，整合了含二聚体的DNA。复制后修复显然使uvr-42受体细胞免于整合二聚体的致死作用，但是复制后修复伴随的重组事件大大减少了供体DNA中紧密相连的遗传标记之间的联系。在recG受体中进行转化DNA的修复（其执行切除修复而不是复制后修复）几乎与在野生型细胞中一样有效。然而，与野生型细胞相比，该受体中的连接只有轻微的变化，甚至根本没有变化。紫外线辐射转化DNA整合区大小的明显减少可能主要是由于较大整合区的复制后修复所致。

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