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首页> 外文期刊>Journal of bacteriology >Immunochemical analysis of respiratory-chain components of micrococcus luteus (lysodeikticus).
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Immunochemical analysis of respiratory-chain components of micrococcus luteus (lysodeikticus).

机译：微球菌（lysodeikticus）呼吸链成分的免疫化学分析。

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摘要

Membrane-bound antigens of the respiratory chain of Micrococcus luteus were analyzed by crossed immunoelectrophoresis after growth of the organism in the presence of 59Fe, the flavin adenine dinucleotide-flavin mononucleotide precursor D-[2-14C]riboflavin, or the heme precursor 5-amino-[4-(14)C]levulinic acid. Using zymograms and procedures of selective extraction in conjunction with autoradiography, it was possible to resolve and partially characterize a number of antigens. Succinate dehydrogenase (EC 1.3.99.1) was shown to possess covalently bound flavin and nonheme iron and was possibly present as a complex with cytochrome. Three other dehydrogenases, namely, NADH dehydrogenase, NAD(P)H dehydrogenase (EC 1.6.99.3), and malate dehydrogenase (EC 1.1.1.37), contained flavin in noncovalent linkage, the NAD(P)H dehydrogenase also possessing nonheme iron. Four other discrete antigens (or antigen complexes) containing both iron and heme centers also resolved, as were two minor immunogens possessing iron as the sole detectable prosthetic group.
机译：在59Fe，黄素腺嘌呤二核苷酸-黄素单核苷酸前体D- [2-14C]核黄素或血红素前体5-羟色胺存在下，在微生物生长后，通过交叉免疫电泳分析微球菌呼吸链的膜结合抗原。氨基-[4-（14）C]乙酰丙酸。使用酶谱图和与放射线照相术结合的选择性提取程序，可以解析和部分表征许多抗原。琥珀酸脱氢酶（EC 1.3.99.1）具有共价键结合的黄素和非血红素铁，可能与细胞色素形成复合物。其他三种脱氢酶，即NADH脱氢酶，NAD（P）H脱氢酶（EC 1.6.99.3）和苹果酸脱氢酶（EC 1.1.1.37），都含有非共价键黄素，NAD（P）H脱氢酶也具有非血红素铁。同时含有铁和血红素中心的其他四个离散抗原（或抗原复合物）也被解析，两个具有铁作为唯一可检测假体的次要免疫原也被分解。

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