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首页> 外文期刊>Journal of bacteriology >Primary structure of the peptidoglycan from the unicellular cyanobacterium Synechocystis sp. strain PCC 6714.
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Primary structure of the peptidoglycan from the unicellular cyanobacterium Synechocystis sp. strain PCC 6714.

机译：单细胞蓝藻集胞藻属（Synechocystis sp。）的肽聚糖的一级结构。株PCC 6714。

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摘要

A peptidoglycan fraction free of non-peptidoglycan components was isolated from the unicellular cyanobacterium Synechocystis sp. strain PCC 6714. Hydrofluoric acid treatment (48%, 0 degrees C, 48 h) cleaved off from the peptidoglycan non-peptidoglycan glucosamine, mannosamine, and mannose. The purified peptidoglycan consists of N-acetyl muramic acid, N-acetyl glucosamine, L-alanine, D-alanine, D-glutamic acid, and meso-diaminopimelic acid in approximately equimolar amounts. At least partial amidation of carboxy groups in the peptide subunits is indicated. Peptide analyses and 2,4-dinitrophenyl studies of partial acid hydrolysates revealed the structure of the Synechocystis sp. strain PCC 6714 peptidoglycan to belong to the A1 gamma type (direct cross-linkage) of peptidoglycan classification. The degree of cross-linkage is about 56% and thus is in the range of that found in gram-positive bacteria. Some of the peptide units are present as tripeptides lacking the carboxy-terminal D-alanine.
机译：从单细胞蓝藻Synechocystis sp。分离出不含非肽聚糖成分的肽聚糖级分。菌株PCC6714。氢氟酸处理（48％，0摄氏度，48小时）从肽聚糖，非肽聚糖葡糖胺，甘露糖胺和甘露糖中分离出来。纯化的肽聚糖由大约等摩尔量的N-乙酰基尿酸，N-乙酰基葡糖胺，L-丙氨酸，D-丙氨酸，D-谷氨酸和内-二氨基庚二酸组成。指出了肽亚基中羧基的至少部分酰胺化。肽分析和部分酸水解产物的2,4-二硝基苯基研究揭示了集胞藻的结构。菌株PCC 6714肽聚糖属于肽聚糖分类的A1γ型（直接交联）。交联度约为56％，因​​此处于革兰氏阳性细菌中。一些肽单元以缺乏羧基末端D-丙氨酸的三肽存在。

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