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首页> 外文期刊>Journal of bacteriology >Nitrogen Source Regulates Glutamate Dehydrogenase NADP Synthesis in Neurospora crassa
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Nitrogen Source Regulates Glutamate Dehydrogenase NADP Synthesis in Neurospora crassa

机译:氮源调节芥菜中谷氨酸脱氢酶NADP的合成

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摘要

Neurospora crassa glutamate dehydrogenase-NADP (EC 1.3.1.3) has a higher activity when mycelium is grown on ammonium or nitrate as nitrogen source than when grown on glutamate or glutamine. Quantitative immunoelectrophoresis established that, under all conditions, enzyme activity corresponded to enzyme concentration. Isotope incorporation studies demonstrated that the nitrogen source exerts its regulation at the level of de novo enzyme synthesis.
机译:菌丝体以铵或硝酸盐为氮源生长时, Neurospora crassa 谷氨酸脱氢酶-NADP(EC 1.3.1.3)的活性高于在谷氨酸或谷氨酰胺中生长的菌丝体。定量免疫电泳证实,在所有条件下,酶活性均对应于酶浓度。同位素掺入研究表明,氮源在从头酶合成水平上发挥其调节作用。

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