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首页> 外文期刊>Journal of bacteriology >Pseudomonas solanacearum genes controlling both pathogenicity on tomato and hypersensitivity on tobacco are clustered.
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Pseudomonas solanacearum genes controlling both pathogenicity on tomato and hypersensitivity on tobacco are clustered.

机译：控制番茄致病性和烟草超敏性的青枯假单胞菌基因聚类。

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摘要

A pLAFR3 cosmid clone designated pVir2 containing a 25-kilobase (kb) DNA insert was isolated from a wild-type Pseudomonas solanacearum GMI1000 genomic library. This cosmid was shown to complement all but one of the nine Tn5-induced mutants which have been isolated after random mutagenesis and which have lost both pathogenicity toward tomato and ability to induce hypersensitive reaction (HR) on tobacco (hrp mutants). The insert is colinear with the genome and provides restoration of the HR-inducing ability when transferred into several Tn5-induced hrp mutants, but failed to complement deletion mutants extending on both sides of the pVir2 region. Localized mutagenesis demonstrated that the hrp genes are clustered within a 17.5-kb region of pVir2 and that this cluster probably extends on the genomic region adjacent to the pVir2 insert. A 3-kb region adjacent to the hrp cluster modulates aggressiveness toward tomato but does not control HR-inducing ability. Sequences within the hrp cluster of pVir2 have homology with the genomic DNA of Xanthomonas campestris strains representing eight different pathovars, suggesting that a set of common pathogenicity functions could be shared by P. solanacearum and X. campestris.
机译：从野生型青枯假单胞菌GMI1000基因组文库中分离出一个pLAFR3粘粒克隆，命名为pVir2，含有25碱基（kb）的DNA插入片段。已显示该粘粒可补充九种由Tn5诱导的突变体中的所有突变体，这些突变体是在随机诱变后分离出的，并且已经失去了对番茄的致病性和诱导烟草过敏反应（HR）的能力（hrp突变体）。该插入片段与基因组共线，当转移到几个Tn5诱导的hrp突变体中时，可恢复HR诱导能力，但无法补充在pVir2区两侧延伸的缺失突变体。局部诱变表明，hrp基因聚簇在pVir2的17.5kb区域内，并且该簇可能在与pVir2插入片段相邻的基因组区域上延伸。与hrp簇相邻的3kb区域可调节对番茄的侵略性，但不能控制HR诱导能力。 pVir2的hrp簇内的序列与代表八个不同病原体的油菜黄单胞菌菌株的基因组DNA具有同源性，这表明青枯菌和油菜X共享了一组共同​​的致病功能。

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