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首页> 外文期刊>Journal of bacteriology >A role for osmotic stress-induced proteins in the osmotolerance of a nitrogen-fixing cyanobacterium, Anabaena sp. strain L-31.
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A role for osmotic stress-induced proteins in the osmotolerance of a nitrogen-fixing cyanobacterium, Anabaena sp. strain L-31.

机译:渗透胁迫诱导的蛋白质在固氮蓝细菌鱼腥藻的渗透耐受中的作用。 L-31株。

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摘要

The molecular basis of tolerance to osmotic stress was investigated with a cyanobacterium, Anabaena sp. strain L-31. The inherent osmotolerance of this strain (50% growth inhibition at 350 mM sucrose) was enhanced by adaptation with 100 mM sucrose for 30 min. Addition of 10 mM KNO3 during growth also conferred significant osmoprotection, but addition of 3 mM NH4Cl did not. Exposure of cells to 350 mM sucrose induced the expression of at least 12 osmotic-stress-induced proteins (OSPs) within 30 min, in the molecular mass range of 11.5 to 84 kDa. Exposure of cells to 100 mM sucrose or to 10 mM nitrate also induced all the OSPs, but addition of ammonium did not. The observed correspondence between the presence of OSPs and osmotolerance strongly suggests a role for OSPs in osmotolerance of Anabaena sp. strain L-31.
机译:用蓝细菌Anabaena sp。研究了对渗透胁迫的耐受性的分子基础。 L-31株。该菌株固有的渗透耐受性(在350 mM蔗糖上抑制50%的生长)通过用100 mM蔗糖适应30分钟而增强。在生长过程中添加10 mM的KNO3也具有显着的渗透保护作用,但添加3 mM的NH4Cl却没有。将细胞暴露于350 mM蔗糖可在30分钟内诱导至少12种渗透压诱导的蛋白(OSP)的表达,分子量范围为11.5至84 kDa。将细胞暴露于100 mM蔗糖或10 mM硝酸盐也可诱导所有OSP,但不添加铵。观察到的OSP的存在与渗透性之间的对应性强烈暗示OSP在鱼腥藻的渗透性中的作用。 L-31株。

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