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首页> 外文期刊>Journal of bacteriology >Genetics of the serine cycle in Methylobacterium extorquens AM1: identification of sgaA and mtdA and sequences of sgaA, hprA, and mtdA.
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Genetics of the serine cycle in Methylobacterium extorquens AM1: identification of sgaA and mtdA and sequences of sgaA, hprA, and mtdA.

机译：甲基芽胞杆菌AM1丝氨酸循环的遗传学：鉴定sgaA和mtdA以及sgaA，hprA和mtdA的序列。

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In a previous paper, we reported identification of the 5' part of hprA of Methylobacterium extorquens AM1, which encodes the serine cycle enzyme hydroxypyruvate reductase (L. V. Chistoserdova and M. E. Lidstrom, J. Bacteriol. 174:71-77, 1992). Here we present the complete sequence of hprA and partial sequence of genes adjacent to hprA. Upstream of hprA, the 3' part of an open reading frame was discovered, separated from hprA by 263 bp. This open reading frame was identified as the gene encoding another serine cycle enzyme, serine glyoxylate aminotransferase (sgaA). Cells containing an insertion mutation into sgaA were unable to grow on C1 compounds, demonstrating that the gene is required for C1 metabolism. Sequencing downstream of hprA has revealed the presence of another open reading frame (mtdA), which is probably cotranscribed with hprA. This open reading frame was identified as the gene required for the synthesis of 5,10-methylenetetrahydrofolate dehydrogenase. Our data suggest that this enzyme plays an integral role in methylotrophic metabolism in M. extorquens AM1, either in formaldehyde oxidation or as part of the serine cycle.
机译：在先前的文章中，我们报道了甲基芽胞杆菌AM1的hprA的5'部分的鉴定，该甲基芽孢菌编码丝氨酸循环酶羟基丙酮酸还原酶（L. V. Chistoserdova和M. E. Lidstrom，细菌学杂志174：71-77，1992）。在这里，我们介绍了hprA的完整序列和与hprA相邻的基因的部分序列。在hprA的上游，发现了一个开放阅读框的3'部分，与hprA隔开263 bp。该开放阅读框被鉴定为编码另一种丝氨酸循环酶丝氨酸乙醛酸转氨酶（sgaA）的基因。含有sgaA插入突变的细胞无法在C1化合物上生长，表明该基因是C1代谢所必需的。 hprA的下游测序揭示了另一个开放阅读框（mtdA）的存在，它可能与hprA共转录。该开放阅读框被鉴定为合成5,10-亚甲基四氢叶酸脱氢酶所需的基因。我们的数据表明，该酶在变形杆菌AM1的甲基营养代谢中起着不可或缺的作用，无论是甲醛氧化还是丝氨酸循环的一部分。

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