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首页> 外文期刊>Journal of bacteriology >Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils.
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Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils.

机译：编码流感嗜血杆菌b型表面原纤维的遗传基因座的表征。

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Haemophilus influenzae is a common gram-negative pathogen that initiates infection by colonizing the upper respiratory tract epithelium. In previous work, we reported the isolation of a locus involved in expression of short, thin surface fibrils by H. influenzae type b and presented evidence that surface fibrils promote attachment to human epithelial cells. In the present study, we determined that the fibril locus is composed of one long open reading frame, designated hsf, which encodes a protein (Hsf) with a molecular mass of approximately 240 kDa. The derived amino acid sequence of the hsf product demonstrated 81% similarity and 72% identity to a recently identified nontypeable H. influenzae adhesin referred to as Hia. In experiments with a panel of eight cultured cell lines, the Hsf and Hia proteins were found to confer the same binding specificities, suggesting that hsf and hia are alleles of the same locus. Southern analysis and mutagenesis studies reinforced this conclusion. Further investigation revealed that an hsf homolog is ubiquitous among encapsulated H. influenzae strains and is present in a subset of nontypeable Haemophilus strains as well. We speculate that the hsf gene product plays an important role in the process of respiratory tract colonization by H. influenzae.
机译：流感嗜血杆菌是一种常见的革兰氏阴性病原体，可通过在上呼吸道上皮定植而引发感染。在以前的工作中，我们报告了b型流感嗜血杆菌分离出一个与表达短而薄的表面原纤维有关的基因座，并提供了证据表明表面原纤维可促进与人上皮细胞的附着。在本研究中，我们确定原纤维基因座由一个长的开放阅读框（称为hsf）组成，该阅读框编码分子质量约为240 kDa的蛋白质（Hsf）。 hsf产品的衍生氨基酸序列与最近鉴定为Hia的不可分型流感嗜血杆菌粘附素显示81％的相似性和72％的同一性。在一组由八种培养的细胞系组成的实验中，发现Hsf和Hia蛋白具有相同的结合特异性，这表明hsf和hia是同一基因座的等位基因。 Southern分析和诱变研究证实了这一结论。进一步的研究表明，hsf同源物在封装的流感嗜血杆菌菌株中无处不在，并且也存在于不可分型嗜血杆菌菌株的子集中。我们推测hsf基因产物在流感嗜血杆菌的呼吸道定殖过程中起着重要作用。

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