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首页> 外文期刊>Journal of bacteriology >An autolysin ring associated with cell separation of Staphylococcus aureus.
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An autolysin ring associated with cell separation of Staphylococcus aureus.

机译：一个自溶素环与金黄色葡萄球菌的细胞分离有关。

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atl is a newly discovered autolysin gene in Staphylococcus aureus. The gene product, ATL, is a unique, bifunctional protein that has an amidase domain and a glucosaminidase domain. It undergoes proteolytic processing to generate two extracellular peptidoglycan hydrolases, a 59-kDa endo-beta-N-acetylglucosaminidase and a 62-kDa N-acetylmuramyl-L-alanine amidase. It has been suggested that these enzymes are involved in the separation of daughter cells after cell division. We recently demonstrated that atl gene products are cell associated (unpublished data). The cell surface localization of the atl gene products was investigated by immunoelectron microscopy using anti-62-kDa N-acetylmuramyl-L-alanine amidase or anti-51-kDa endo-beta-N-acetylglucosaminidase immunoglobulin G. Protein A-gold particles reacting with the antigen-antibody complex were found to form a ring structure on the cell surface at the septal region for the next cell division site. Electron microscopic examination of an ultrathin section of the preembedded sample revealed preferential distribution of the gold particles at the presumptive sites for cell separation where the new septa had not been completed. The distribution of the gold particles on the surface of protoplast cells and the association of the gold particles with fibrous materials extending from the cells suggested that some atl gene products were associated with a cellular component extending from the cell membrane, such as lipoteichoic acid. The formation of a ring structure of atl gene products may be required for efficient partitioning of daughter cells after cell division.
机译：atl是在金黄色葡萄球菌中新发现的自溶素基因。基因产物ATL是一种独特的双功能蛋白，具有一个酰胺酶结构域和一个氨基葡萄糖苷酶结构域。它经历了蛋白水解过程，产生了两个细胞外肽聚糖水解酶，一个59 kDa的内-β-N-乙酰氨基葡糖苷酶和一个62 kDa的N-乙酰村酰-L-丙氨酸酰胺酶。已经建议这些酶参与细胞分裂后子细胞的分离。我们最近证明了atl基因产物与细胞相关（未发表的数据）。通过使用抗62-kDa N-乙酰村mura基-L-丙氨酸酰胺酶或抗51-kDa内-β-N-乙酰氨基葡糖苷酶免疫球蛋白G的免疫电子显微镜研究了atl基因产物的细胞表面定位。蛋白A-金颗粒反应发现具有抗原-抗体复合物的N-氨基乙酰胺在下一个细胞分裂位点的间隔区域的细胞表面上形成环结构。对预埋样品的超薄切片进行电子显微镜检查后发现，金颗粒优先分布在未完成新隔垫的细胞分离假定位置。金颗粒在原生质体细胞表面的分布以及金颗粒与从细胞延伸的纤维材料的缔合表明某些atl基因产物与从细胞膜延伸的细胞成分（如脂磷壁酸）相关。细胞分裂后有效分配子细胞可能需要atl基因产物的环结构的形成。

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