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首页> 外文期刊>Journal of bacteriology >Use of reduced sulfur compounds by Beggiatoa sp.
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Use of reduced sulfur compounds by Beggiatoa sp.

机译：Beggiatoa sp。使用还原的硫化合物。

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A strain of Beggiatoa cf. leptomitiformis (OH-75-B, clone 2a) was isolated which is unique among reported strains in its ability to deposit internal sulfur granules from thiosulfate. It also deposited these characteristic granules (as all BEggiatoa species do) from sulfide. In cultures where growth was limited by exhaustion of organic substrates, these granules generally comprised about 20% of the total cell weight. With medium containing acetate and thiosulfate, no measurable utilization of thiosulfate or deposition of elemental sulfur (S0) took place until after the exponential growth phase. Neither sulfide nor thiosulfate added an increment to heterotrophic growth yield except for the weight of the deposited S0. The deposition of S0 from thiosulfate was probably a disproportionation in which S0 and sulfate were produced in a 1:1 ratio. Some of the S0 was further oxidized to sulfate. No autotrophic or mixotrophic growth was demonstrated for this strain. When inoculated in small, well-dispersed quantities into yeast extract medium, this strain grew only after long lags. Addition of the enzyme catalase eliminated initial lags and increased growth rates slightly. In contrast, catalase had no influence on growth rate when added to mineral medium containing acetate. In yeast extract medium, the inhibition of growth rate was presumably because of peroxides. Addition of thiosulfate was almost as effective as catalase in eliminating this inhibition. The S0 granules which, in this case, were deposited during the exponential growth phase, appeared to be partly responsible for this relief. This strain of Beggiatoa sp. remained active for at least 5 days under strictly anaerobic conditions, and under those conditions, it increased its dry weight by about 2.5-fold. Anaerobic "growth" and maintenance required the presence of an energy source, such as acetate. When cells containing much internal S0 were transferred to an organic anaerobic medium, a substantial portion of the internal S0 was eventually converted to sulfide.
机译：Beggiatoa菌株分离了钩端螺旋体（OH-75-B，克隆2a），这在所报道的菌株中是独特的，其能够从硫代硫酸盐沉积内部硫颗粒的能力。它还从硫化物中沉积了这些特征性颗粒（就像所有贝格托亚物种一样）。在受有机底物耗尽限制生长的培养物中，这些颗粒通常占细胞总重量的约20％。在含有乙酸盐和硫代硫酸盐的培养基中，直到指数生长阶段之后，才发生可测量的硫代硫酸盐利用或元素硫（S0）的沉积。除了沉积的SO的重量，硫化物和硫代硫酸盐都没有增加异养生长的产率。来自硫代硫酸盐的S0沉积可能是歧义，其中S0和硫酸盐以1：1的比例产生。一些SO进一步被氧化为硫酸盐。该菌株没有自养或杂养生长。当将少量，分散良好的疫苗接种到酵母提取液中时，该菌株仅在长时间滞后后才生长。过氧化氢酶的加入消除了最初的滞后，并稍微提高了生长速度。相反，过氧化氢酶在添加到含乙酸盐的矿物培养基中时对生长速率没有影响。在酵母提取物培养基中，生长速率的抑制可能是由于过氧化物。在消除这种抑制作用方面，硫代硫酸盐的添加几乎与过氧化氢酶一样有效。在这种情况下，S0颗粒在指数生长期沉积，似乎是造成这种缓解的部分原因。 Beggiatoa sp。的这种菌株。在严格的厌氧条件下保持活性至少5天，在这些条件下，其干重增加了约2.5倍。厌氧的“生长”和维持需要能量源的存在，例如乙酸盐。当将含有大量内部SO的细胞转移到有机厌氧培养基中时，内部SO的大部分最终会转化为硫化物。

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