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首页> 外文期刊>Journal of bacteriology >Genetic analysis of mutants affected in the Pst inorganic phosphate transport system.
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Genetic analysis of mutants affected in the Pst inorganic phosphate transport system.

机译：在Pst无机磷酸盐转运系统中受影响的突变体的遗传分析。

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A number of mutant alleles affecting the Pst phosphate transport system have been divided into three complementation groups on the basis of constitutive alkaline phosphatase activity in appropriate partial diploid strains. The three complementation groups were represented by the alleles pstA2 and phoT32 and the newly described allele pstB401. The two alleles phoS28 and phoS21 appeared to be polar. The phoS28 allele affected both the phoT and pstB genes but not the pstA gene, whereas the phoS21 allele appeared to be a mutation in the pstA gene exerting polar effects on both the pstB and phoT genes. It was concluded that the three genes pstA, pstB, and phoT were part of an operon and that the phosphate-binding protein was not coded for by any of these genes. The phoS gene, defined as the structural gene for the phosphate-binding protein, is also part of the operon, but the phoS28 and phoS21 alleles are not mutations in the phoS gene and were reclassified as pho-28 and pho-21 alleles. The gene order was concluded to be pstA-(pstB-phoT)-phoS, with the pstA gene promotor proximal and the direction of transcription opposite to that of the nearby unc operon.
机译：根据适当的部分二倍体菌株中的组成型碱性磷酸酶活性，许多影响Pst磷酸转运系统的突变等位基因已分为三个互补组。三个互补组由等位基因pstA2和phoT32以及新描述的等位基因pstB401代表。两个等位基因phoS28和phoS21似乎是极性的。 phoS28等位基因影响phoT和pstB基因，但不影响pstA基因，而phoS21等位基因似乎是pstA基因中的突变，对pstB和phoT基因均产生极性影响。可以得出结论，三个基因pstA，pstB和phoT是操纵子的一部分，并且这些基因中的任何一个都不编码磷酸盐结合蛋白。 phoS基因（被定义为磷酸结合蛋白的结构基因）也是操纵子的一部分，但phoS28和phoS21等位基因不是phoS基因的突变，被重新分类为pho-28和pho-21等位基因。该基因顺序被推断为pstA-（pstB-phoT）-phoS，pstA基因启动子近端，转录方向与附近的unc操纵子相反。

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