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首页> 外文期刊>Journal of bacteriology >The cell-bound fructosyltransferase of Streptococcus salivarius: the carboxyl terminus specifies attachment in a Streptococcus gordonii model system.
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The cell-bound fructosyltransferase of Streptococcus salivarius: the carboxyl terminus specifies attachment in a Streptococcus gordonii model system.

机译：唾液链球菌的细胞结合果糖基转移酶：羧基末端指定了戈登链球菌模型系统中的附件。

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摘要

The ftf gene, coding for the cell-bound beta-D-fructosyltransferase (FTF) of Streptococcus salivarius ATCC 25975, has been analyzed, and its deduced amino acid sequence has been compared with that of the secreted FTF of Streptococcus mutans and the levansucrases (SacBs) of Bacillus species. A unique proline-rich region detected at the C terminus of the FTF of S. salivarius preceded a hydrophobic terminal domain. This proline-rich region was shown to possess strong homology to the product of the prgC gene from pCF10 in Enterococcus faecalis, which encodes a pheromone-responsive protein of unknown function, as well as homology to the human proline-rich salivary protein PRP-4. A series of 3'-OH deletions of the S. salivarius ftf gene expressed in Streptococcus gordonii Challis LGR2 showed that the C terminus was required for cell surface attachment in this heterologous organism, as only the complete gene product was cell bound. This cell-bound activity was released in the presence of sucrose, suggesting that the mode of attachment and release of the S. salivarius FTF in S. gordonii was similar to that in its native host.
机译：已经分析了ftf基因，该基因编码唾液链球菌ATCC 25975的细胞结合β-D-果糖基转移酶（FTF），并将其推导的氨基酸序列与变形链球菌和Levansucrases分泌的FTF的氨基酸序列进行了比较（ SacBs）。在唾液链球菌FTF的C末端检测到的独特的富含脯氨酸的区域位于疏水性末端结构域之前。该富含脯氨酸的区域显示出与粪肠球菌pCF10的prgC基因的产物具有强烈的同源性，该产物编码未知功能的信息素响应蛋白，并且与人类富含脯氨酸的唾液蛋白PRP-4同源。 。在戈登链球菌查利斯LGR2中表达的唾液链球菌ftf基因的一系列3'-OH缺失显示，在此异源生物中，细胞表面附着需要C末端，因为只有完整的基因产物才与细胞结合。这种细胞结合的活性在蔗糖存在下释放，这表明戈登酵母中唾液链球菌FTF的附着和释放模式与其天然宿主相似。

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