Heterologous expression of the bchM gene product from Rhodobacter capsulatus and demonstration that it encodes S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase.

D W Bollivar; Z Y Jiang; C E Bauer; S I Beale

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Heterologous expression of the bchM gene product from Rhodobacter capsulatus and demonstration that it encodes S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase.

机译：荚膜红细菌bchM基因产物的异源表达，并证明其编码S-腺苷-L-蛋氨酸：Mg-原卟啉IX甲基转移酶。

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The bacteriochlorophyll biosynthesis gene, bchM, from Rhodobacter capsulatus was previously believed to code for a polypeptide involved in formation of the cyclopentone ring of protochlorophyllide from Mg-protoporphyrin IX monomethyl ester. In this study, R. capsulatus bchM was expressed in Escherichia coli and the gene product was subsequently demonstrated by enzymatic analysis to catalyze methylation of Mg-protoporphyrin IX to form Mg-protoporphyrin IX monomethyl ester. Activity required the substrates Mg-protoporphyrin IX and S-adenosyl-L-methionine. 14C-labeled product was formed in incubations containing 14C-methyl-labeled S-adenosyl-L-methionine. On the basis of these and previous results, we also conclude that the bchH gene, which was previously reported to code for Mg-protoporphyrin IX methyltransferase, is most likely involved in the Mg chelation step.

机译：以前认为来自荚膜红细菌的细菌叶绿素生物合成基因bchM编码一种多肽，该多肽参与了由Mg-原卟啉IX单甲酯形成原叶绿素环戊酮环的过程。在这项研究中，荚膜红衣菌bchM在大肠杆菌中表达，随后通过酶促分析证明基因产物催化Mg-原卟啉IX甲基化形成Mg-原卟啉IX单甲基酯。活性需要底物Mg-原卟啉IX和S-腺苷-L-蛋氨酸。在含有14C-甲基标记的S-腺苷-L-蛋氨酸的培养液中形成14C标记的产物。根据这些结果和先前的结果，我们还得出结论，以前据报道编码Mg-原卟啉IX甲基转移酶的bchH基因很可能参与了Mg螯合步骤。

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《Journal of bacteriology》 |1994年第17期|共7页
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D W Bollivar; Z Y Jiang; C E Bauer; S I Beale;
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1. Heterologous expression of the bchM gene product from Rhodobacter capsulatus and demonstration that it encodes S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase. [J] . D W Bollivar, Z Y Jiang, C E Bauer, Journal of bacteriology . 1994,第17期

机译：荚膜红细菌bchM基因产物的异源表达，并证明其编码S-腺苷-L-蛋氨酸：Mg-原卟啉IX甲基转移酶。
2. The bacteriochlorophyll biosynthesis gene, bchM, of Rhodobacter sphaeroides encodes S‐adenosyl‐L‐methionine: Mg protoporphyrin IX methyltransferase [J] . Gibson Lucien C.D., Hunter C.Neil FEBS Letters . 1994,第2期

机译：球形球形红细菌的细菌叶绿素生物合成基因bchM编码S-腺苷-L-蛋氨酸：Mg原卟啉IX甲基转移酶
3. S-Adenosyl-L-methionine:magnesium-protoporphyrin IX O-methyltransferase from Rhodobacter capsulatus: mechanistic insights and stimulation with phospholipids [J] . Artur Sawicki, Robert?D. Willows The biochemical journal . 2007,第3期

机译：S-腺苷-L-蛋氨酸：来自荚膜红细菌的镁-原卟啉IX O-甲基转移酶：机理的见解和磷脂的刺激
4. EXPRESSION OF 3-VINYL BACTERIOCHLOROPHYLLIDE HYDRATASE bchF FROM PURPLE NON-SULFUR BACTERIUM RHODOBACTER CAPSULATUS AND GREEN SULFUR BACTERIUM CHLOROBIUM TEPIDUMIN CYANOBACTERIUM SYNECHOCYSTIS SP. PCC6803 [C] . Tomoyuki Seki, Masahiro Atsuta, Yosuke Hasimoto, International Congress of Photosynthesis . 2005

机译：3-乙烯基氯化氯化物水分BCHF的表达来自紫色非硫磺菌岩岩和绿硫菌氯钴氯酸钴酸纤维杆菌综合症SP。 PCC6803.
5. Mapping nif genes of Rhodobacter capsulatus, and characterization of a mutant with a pleiotropic defect in nitrogen metabolism. [D] . Goldenberg, Ann. 1988

机译：映射荚膜红细菌的nif基因，并表征氮代谢中具有多效性缺陷的突变体。
6. Heterologous expression of the bchM gene product from Rhodobacter capsulatus and demonstration that it encodes S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase. [O] . D W Bollivar, Z Y Jiang, C E Bauer, 1994

机译：来自荚膜红细菌的bchM基因产物的异源表达并证明它编码S-腺苷-L-蛋氨酸：Mg-原卟啉IX甲基转移酶。
7. Heterologous expression of the bchM gene product from Rhodobacter capsulatus and demonstration that it encodes S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase. [O] . Bollivar, D W, Jiang, Z Y, Bauer, C E, 1994

机译：来自荚膜红细菌的bchM基因产物的异源表达，并证明它编码S-腺苷-L-蛋氨酸：Mg-原卟啉IX甲基转移酶。
8. Molecular genetic and molecular evolutionary studies on the bacteriochlorophyll synthesis genes of Rhodobacter capsulatus [R] . Burke-Agueero, D H 1992

机译：荚膜红细菌（Rhodobacter capsulatus）细菌叶绿素合成基因的分子遗传学和分子进化研究

Heterologous expression of the bchM gene product from Rhodobacter capsulatus and demonstration that it encodes S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase.

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