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首页> 外文期刊>Journal of bacteriology >Identification of VCR, a repeated sequence associated with a locus encoding a hemagglutinin in Vibrio cholerae O1.
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Identification of VCR, a repeated sequence associated with a locus encoding a hemagglutinin in Vibrio cholerae O1.

机译:VCR的鉴定,VCR是与霍乱弧菌O1中编码血凝素的基因座相关的重复序列。

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摘要

We have determined the nucleotide sequence of a 6.3-kb BamHI fragment of the chromosome of Vibrio cholerae 569B that includes the sequence of the mannose-fucose-resistant hemagglutinin reported previously (V.L. Franzon, A. Barker, and P. A. Manning, Infect. Immun. 61:3032-3037, 1993). This region contains nine copies of a 124-bp direct repeat, here named VCR, of imperfect dyad symmetry, that are shown by Southern hybridization to occur at least 60 to 100 times in the V. cholerae O1 chromosome. Large-scale chromosomal mapping suggests that the repeats are confined to about 10% of the chromosome. Related sequences are also found in non-O1 V. cholerae but not in other members of the family Vibrionaceae. However, VCR is unrelated to other previously described repetitive sequences.
机译:我们已经确定了霍乱弧菌569B染色体的6.3-kb BamHI片段的核苷酸序列,该序列包括先前报道的抗甘露糖-岩藻糖抗性血凝素的序列(VL Franzon,A.Barker和PA Manning,Infect。Immun。 61:3032-3037,1993)。该区域包含九个拷贝的不完整双胞胎对称性的124 bp直接重复(此处称为VCR)的拷贝,Southern杂交显示在霍乱弧菌O1染色体中发生了至少60至100次。大规模染色体作图表明重复序列仅限于染色体的10%。在非O1霍乱弧菌中也发现了相关序列,但在弧菌科的其他成员中未发现相关序列。但是,VCR与其他先前描述的重复序列无关。

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