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首页> 外文期刊>Journal of bacteriology >Gin mutants that can be suppressed by a Fis-independent mutation.
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Gin mutants that can be suppressed by a Fis-independent mutation.

机译：可以被Fis独立突变抑制的Gin突变体。

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摘要

The Gin invertase of bacteriophage Mu mediates recombination between two inverted gix sites. Recombination requires the presence of a second protein, Fis, which binds to an enhancer sequence. We have isolated 24 different mutants of Gin that are impaired in DNA inversion but proficient in DNA binding. Six of these mutants could be suppressed for inversion by introduction of a second mutation, which when present in the wild-type gin gene causes a Fis-independent phenotype. Only one of the six resulting double mutants shows an inversion efficiency which is comparable to that of the wild-type Gin and which is independent of Fis. The corresponding mutation, M to I at position 108 (M108I), is located in a putative alpha-helical structure, which in the homologous gamma delta resolvase has been implicated in dimerization. The properties of the M108I mutant suggest that in Gin this dimerization helix might also be the target for Fis interaction. The five other mutants that show a restored inversion after introduction of a Fis-independent mutation appear to be completely dependent on Fis for this inversion. The corresponding mutations are located in different domains of the protein. The properties of these mutants in connection with the role of Fis in inversion will be discussed.
机译：噬菌体Mu的Gin转化酶介导两个反向gix位点之间的重组。重组需要存在第二个蛋白Fis，该蛋白与增强子序列结合。我们已经分离了24种不同的Gin突变体，这些突变体在DNA转化中受损但在DNA结合方面很熟练。通过引入第二个突变可以抑制其中的六个突变体的倒置，当第二个突变存在于野生型gin基因中时，会导致Fis非依赖性表型。结果产生的六个双突变体中只有一个具有与野生型Gin相当的转化效率，并且与Fis无关。位置108（M108I）处的M到I对应突变位于推定的α-螺旋结构中，该结构在同源的γ-δ分辨酶中与二聚化有关。 M108I突变体的特性表明，在杜松子酒中，这种二聚化螺旋也可能是Fis相互作用的目标。引入Fis非依赖性突变后，其他五个显示突变恢复的突变体似乎完全依赖于Fis进行这种转化。相应的突变位于蛋白质的不同结构域。这些突变体的性质与Fis在反演中的作用有关。

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