Implication of nifA in regulation of genes located on a Rhizobium meliloti cryptic plasmid that affect nodulation efficiency.

J Sanjuan; J Olivares

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Implication of nifA in regulation of genes located on a Rhizobium meliloti cryptic plasmid that affect nodulation efficiency.

机译：nifA在调节根瘤菌隐性质粒上影响结瘤效率的基因调控中的意义。

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We examined the contribution of a cryptic plasmid, pRmeGR4b, to the nodulation of Medicago sativa by strain GR4 of Rhizobium meliloti. A 905-base-pair PstI DNA fragment in pRmeGR4b was found to hybridize DNA of the R. meliloti fixA promoter region as a probe. Sequence analysis of the PstI fragment showed a 206-base-pair region displaying high homology with the DNA upstream of the RNA start points of the P1 and P2 symbiotic promoters. Putative nif promoter consensus sequences were conserved in this DNA segment. Expression of DNA downstream of the nif promoterlike sequence, monitored by beta-galactosidase activity of different lacZ fusions, was demonstrated to depend on a functional nifA gene, both in microaerobically free-living cells and in nodules. Individual transposon Tn3-HoHo1 insertions in this DNA region caused a reduced nodulation competitiveness. This new symbiotic region, occupying approximately 5 kilobases of pRmeGR4b DNA, was called nfe (nodule formation efficiency).

机译：我们研究了隐性质粒pRmeGR4b对苜蓿根瘤菌菌株GR4对紫花苜蓿结瘤的贡献。发现pRmeGR4b中的一个905个碱基对的PstI DNA片段可杂交苜蓿根瘤菌fixA启动子区域的DNA作为探针。 PstI片段的序列分析显示一个206个碱基对的区域与P1和P2共生启动子的RNA起点上游的DNA表现出高度同源性。推定的nif启动子共有序列在该DNA片段中保守。通过不同的lacZ融合的β-半乳糖苷酶活性监测，nif启动子样序列下游DNA的表达在微需氧自由活动细胞和结节中均依赖于功能性nifA基因。单个转座子Tn3-HoHo1插入此DNA区域导致结瘤竞争性降低。这个新的共生区大约占pRmeGR4b DNA的5千个碱基，称为nfe（结节形成效率）。

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《Journal of bacteriology》 |1989年第8期|共8页
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J Sanjuan; J Olivares;
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1. Two gene clusters of Rhizobium meliloti code for early essential nodulation functions and a third influences nodulation efficiency. [J] . P Putnoky, A Kondorosi Journal of bacteriology . 1986,第3期

机译：用于早期基本染色功能的三种根瘤莫里洛语码的两个基因簇，第三种影响促进效率。
2. Transcription patterns of Rhizobium meliloti symbiotic plasmid pSym: identification of nifA-independent fix genes. [J] . M David, O Domergue, P Pognonec, Journal of bacteriology . 1987,第5期

机译：苜蓿根瘤菌共生质粒pSym的转录模式：鉴定nifA独立的固定基因。
3. Rhizobium meliloti regulatory gene fixJ activates transcription of R. meliloti nifA and fixK genes in Escherichia coli. [J] . C Hertig, R Y Li, A M Louarn, Journal of bacteriology . 1989,第3期

机译：根瘤菌调控基因fixJ激活大肠杆菌中melothti nifA nixA和fixK基因的转录。
4. GLOBAL CHANGES IN GENE EXPRESSION OF S. meliloti 1021 NODULE BACTERIA IN nifA AND nifti MUTANT BACKGROUND [C] . Zhe-Xian Tian, Hua-Song Zou, Jian Li, International Nitrogen Fixation Congress . 2005

机译：NiFa和NIFI突变背景中S. meliloti 1021结核细菌基因表达的全局变化
5. The roles of flavonoids in the nodulation of alfalfa by Rhizobium meliloti: The effect of R. meliloti on chalcone synthase and chalcone isomerase gene expression patterns. [D] . McKhann, Heather Ileana. 1993

机译：黄酮类化合物在苜蓿根瘤菌根瘤中的作用：苜蓿根瘤菌对查尔酮合酶和查尔酮异构酶基因表达模式的影响。
6. Implication of nifA in regulation of genes located on a Rhizobium meliloti cryptic plasmid that affect nodulation efficiency. [O] . J Sanjuan, J Olivares 1989

机译：nifA在调节根瘤菌隐性质粒上影响结瘤效率的基因调控中的意义。
7. Implication of nifA in regulation of genes located on a Rhizobium meliloti cryptic plasmid that affect nodulation efficiency. [O] . Sanjuan, J, Olivares, J 1989

机译：nifA在调节根瘤菌隐性质粒上影响结瘤效率的基因调控中的意义。
8. Genetics and Biochemistry of Rhizobium Meliloti Acidic Extracellular Heteropolysaccharide and Its Role in Nodulation: Final Technical Report. [R] . Leigh, J. A. 1989

机译：苜蓿根瘤菌酸性细胞外杂多糖的遗传学和生物化学及其在结瘤中的作用：最终技术报告。

Implication of nifA in regulation of genes located on a Rhizobium meliloti cryptic plasmid that affect nodulation efficiency.

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