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首页> 外文期刊>Journal of bacteriology >Rhizobium japonicum mutant strains unable to grow chemoautotrophically with H2.
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Rhizobium japonicum mutant strains unable to grow chemoautotrophically with H2.

机译：日本根瘤菌突变株无法与H2化学自养生长。

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摘要

Rhizobium japonicum strain SR grows chemoautotrophically on a mineral salts medium when incubated in an H2- and CO2-containing atmosphere. Mutant strains unable to grow or that grow very poorly chemoautotrophically with H2 have been isolated from strain SR. The mutant isolation procedure involved mutagenesis with ethyl methane sulfonate, penicillin selection under chemoautotrophic growth conditions, and plating of the survivors onto medium containing carbon. The resulting colonies were replica plated onto medium that did not contain carbon, and the plates were incubated in an H2- and CO2-containing atmosphere. Mutant strains unable to grow under these conditions were chosen. Over 100 mutant strains with defects in chemoautotrophic metabolism were obtained. The phenotypes of the mutants fall into various classes. These include strains unable to oxidize H2 and strains deficient in CO2 uptake. Some of the mutant strains were capable of oxidizing H2 only when artificial electron acceptors were provided. Two mutant strains specifically lack activity of the key CO2-fixing enzyme ribulose 1,5-bisphosphate carboxylase. Other mutant strains lack both H2-oxidizing ability and ribulose 1,5-bisphosphate carboxylase activity.
机译：当在含H2和CO2的气氛中孵育时，日本根瘤菌菌株SR在矿物盐培养基上化学自养生长。已从SR菌株中分离出无法生长或与H2化学自养能力非常弱的突变菌株。突变体分离步骤包括用甲烷磺酸乙酯诱变，在化学自养生长条件下选择青霉素，以及将幸存者接种到含有碳的培养基上。将得到的菌落复制平板接种到不含碳的培养基上，并将平板在含H2和CO2的气氛中孵育。选择在这些条件下不能生长的突变株。获得了超过100种化学自养代谢缺陷的突变菌株。突变体的表型分为各种类别。这些包括无法氧化H2的菌株和缺乏吸收CO2的菌株。仅当提供了人工电子受体时，某些突变菌株才能够氧化H2。两个突变株特别缺乏关键的CO2固定酶核糖1,5-双磷酸羧化酶的活性。其他突变菌株既缺乏H2氧化能力，又缺乏核糖1,5-双磷酸羧化酶活性。

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