Requirement of a heat-labile factor(s) for in vitro expression of the amp gene of pBR322.

Y Kuriki

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Requirement of a heat-labile factor(s) for in vitro expression of the amp gene of pBR322.

机译：热不稳定因子对体外表达pBR322 amp基因的要求。

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The amp gene of pBR328 and pBR322 was expressed less efficiently at 45 than at 30 degrees C in an in vitro coupled transcription-translation system of Escherichia coli. Preincubation of S30 extract at 45 degrees C reduced specifically its ability to express the amp gene at 30 degrees C, indicating inactivation of a factor(s) required for efficient expression of the amp gene.

机译：在大肠杆菌的体外偶联转录-翻译系统中，pBR328和pBR322的amp基因在45℃下的表达效率比30℃下低。 S30提取物在45摄氏度下的预温育会特别降低其在30摄氏度下表达amp基因的能力，这表明该amp基因有效表达所需的因子失活了。

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《Journal of bacteriology》 |1987年第12期|共3页
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Y Kuriki;
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中图分类微生物学;
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5. Effects of pro-inflammatory cytokines on in vitro measures of gene expression, protein expression, and process extension in primary hippocampal neurons. [D] . McLinden, Kristina Ashley. 2011

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6. Requirement of a heat-labile factor(s) for in vitro expression of the amp gene of pBR322. [O] . Y Kuriki 1987

机译：热不稳定因子对体外表达pBR322 amp基因的要求。
7. Requirement of a heat-labile factor(s) for in vitro expression of the amp gene of pBR322. [O] . Kuriki, Y 1987

机译：热不稳定因子对体外表达pBR322 amp基因的要求。

Requirement of a heat-labile factor(s) for in vitro expression of the amp gene of pBR322.

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